Transcriptome Analysis Reveals Candidate Genes Related to Anthocyanin Biosynthesis in Different Carrot Genotypes and Tissues

Geng MengSøren K Rasmussen


Black carrots are characterized by a significant amount of anthocyanins, which are not only a good source of natural food colorant, but can also provide many health benefits to humans. In the present work, taproots of different carrot genotypes were used to identify the candidate genes related to anthocyanin synthesis, with particular a focus on R2R3MYB, bHLH transcription factors, and glutathione S-transferase gene (GST). The RNA-sequencing analysis (RNA-Seq) showed that DcMYB6 and DcMYB7 had a genotypic dependent expression and they are likely involved in the regulation of anthocyanin biosynthesis. They were specifically upregulated in solid black taproots, including both black phloem and xylem. DcbHLH3 (LOC108204485) was upregulated in all black samples compared with the orange ones. We also found that GST1 (LOC108205254) might be an important anthocyanin transporter, and its upregulated expression resulted in the increasing of vacuolar anthocyanin accumulation in black samples. Moreover, high performance liquid chromatographic (HPLC) analysis and liquid chromatography coupled to mass spectrometry (LC-MS) were used to identify the individual anthocyanin in the purple tissues of two carrot cultivars. The results showed that f...Continue Reading


Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K C ConeB Burr
Nov 1, 1995·The Plant Journal : for Cell and Molecular Biology·B W ShirleyH M Goodman
Oct 13, 2001·Current Opinion in Plant Biology·R StrackeB Weisshaar
Oct 3, 2002·Journal of Agricultural and Food Chemistry·Florian C StintzingRonald E Wrolstad
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Satoshi KitamuraAtsushi Tanaka
May 11, 2005·Trends in Plant Science·Ronald KoesFrancesca Quattrocchio
Aug 4, 2005·Journal of Agricultural and Food Chemistry·Anne C KurilichJanet A Novotny
Oct 3, 2006·Plant Physiology·Adam M TakosAmanda R Walker
Dec 22, 2006·The Plant Journal : for Cell and Molecular Biology·Richard V EspleyAndrew C Allan
Feb 19, 2008·Trends in Plant Science·Andrew C AllanWilliam A Laing
Jan 19, 2012·Journal of Plant Physiology·Satoshi KitamuraAtsushi Tanaka
Mar 26, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mehtap YildizPhilipp W Simon
Jul 23, 2013·Trends in Plant Science·Laura Jaakola
Jan 13, 2015·Trends in Plant Science·Wenjia XuLoïc Lepiniec
Aug 12, 2015·Journal of Agricultural and Food Chemistry·Senem KamilogluJohn Van Camp
May 31, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Florencia BannoudPablo F Cavagnaro
Feb 22, 2020·Frontiers in Plant Science·Julien CurabaMassimo Iorizzo

Related Concepts

Mass Spectrometry
Sequence Analysis, RNA
Daucus carota
Basic Helix-Loop-Helix Transcription Factors
High Pressure Liquid Chromatography Procedure
Orange (Fruit)
Fusconaia subrotunda

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.