Transcriptome Analysis Reveals Differentially Expressed Genes and Long Non-coding RNAs Associated With Fecundity in Sheep Hypothalamus With Different FecB Genotypes.

Frontiers in Cell and Developmental Biology
Si ChenMingxing Chu

Abstract

Small-tailed Han sheep, with different FecB genotypes, manifest distinct ovulation rates and fecundities, which are due to differences in reproductive hormones secreted by the hypothalamic-pituitary-ovarian axis. Nevertheless, the function of the hypothalamus against a FecB mutant background on increasing ovulation rate is rarely reported. Therefore, we determined the expression profiles of hypothalamus tissue collected from six wild-type (WW) and six FecB mutant homozygous (BB) ewes at the follicular and luteal phases by whole-transcriptome sequencing. We identified 53 differentially expressed mRNAs (DEGs) and 40 differentially expressed long non-coding RNAs (DELs) between the two estrus states. Functional annotation analysis revealed that one of the DEGs, PRL, was particularly enriched in the hypothalamic function, hormone-related, and reproductive pathways. The lncRNA-target gene interaction networks and KEGG analysis in combination suggest that the lncRNAs LINC-676 and WNT3-AS cis-acting on DRD2 and WNT9B in different phases may induce gonadotropin-releasing hormone (GnRH) secretion. Furthermore, there were differences of regulatory elements and WNT gene family members involved in the follicular-luteal transition in the rep...Continue Reading

References

Feb 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Morrison-Graham, Y Takahashi
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Feb 26, 2000·Journal of Reproduction and Fertility·E DíazB Díaz López
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·P MulsantJ M Elsen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 17, 2003·International Review of Cytology·Zvi YaronBerta Levavi-Sivan
Oct 20, 2005·Nature Neuroscience·Hirokazu HiraiJames I Morgan
Aug 19, 2007·Journal of Cell Science·Alistair N HumeMiguel C Seabra
Jan 29, 2008·Neuroendocrinology·Charles E RoselliFred Stormshak
Nov 29, 2008·Molecular Human Reproduction·Hong-Xing WangGerald M Kidder
Jun 16, 2009·Neuropharmacology·Hans-Christian PapeRainer K Reinscheid
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Feb 19, 2015·Nature Biotechnology·Mihaela PerteaSteven L Salzberg
Feb 8, 2016·The Lancet. Diabetes & Endocrinology·Ana Paula Abreu, Ursula B Kaiser
Mar 5, 2016·Biology of Reproduction·Maoliang RanZhaohui Li
Aug 26, 2016·Nature Protocols·Mihaela PerteaSteven L Salzberg
Jan 10, 2018·Frontiers in Endocrinology·Amy T DesaulniersBrett R White
Apr 11, 2019·Animals : an Open Access Journal From MDPI·Zhuangbiao ZhangMingxing Chu

❮ Previous
Next ❯

Software Mentioned

clusterProfiler
HTSeq
HiSAT
pheatmap
R
HTSeq Python
Pfam
Volcano
CPAT CPAT
bcl2fastq

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.