Transcriptome analysis reveals response regulator SO2426-mediated gene expression in Shewanella oneidensis MR-1 under chromate challenge.

BMC Genomics
Karuna ChoureyDorothea K Thompson

Abstract

Shewanella oneidensis MR-1 exhibits diverse metal ion-reducing capabilities and thus is of potential utility as a bioremediation agent. Knowledge of the molecular components and regulatory mechanisms dictating cellular responses to heavy metal stress, however, remains incomplete. In a previous work, the S. oneidensis so2426 gene, annotated as a DNA-binding response regulator, was demonstrated to be specifically responsive at both the transcript and protein levels to acute chromate [Cr(VI)] challenge. To delineate the cellular function of SO2426 and its contribution to metal stress response, we integrated genetic and physiological approaches with a genome-wide screen for target gene candidates comprising the SO2426 regulon. Inactivation of so2426 by an in-frame deletion resulted in enhanced chromate sensitivity and a reduced capacity to remove extracellular Cr(VI) relative to the parental strain. Time-resolved microarray analysis was used to compare transcriptomic profiles of wild-type and SO2426-deficient mutant S. oneidensis under conditions of chromate exposure. In total, 841 genes (18% of the arrayed genome) were up- or downregulated at least twofold in the Deltaso2426 mutant for at least one of six time-point conditions. Hi...Continue Reading

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Feb 25, 2009·Applied Microbiology and Biotechnology·Irina MugerfeldDorothea K Thompson
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Datasets Mentioned

BETA
24348419
16131752
147675245
16131282
147673571
GSE12129

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Plot
Hierarchical Clustering Explorer ( HCE )
[UNK]
Primer3
Sigma
ClustalW

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