Transcriptome analysis reveals the potential contribution of long noncoding RNAs to brown adipocyte differentiation

Molecular Genetics and Genomics : MGG
Liang Hui YouXirong Guo

Abstract

Brown adipose tissue (BAT) functions to dissipate energy in response to cold exposure or overfeeding. Counteracting obesity has been extensively considered as a promising target. Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. However, the potential biological functions of lncRNAs during mouse brown fat cell differentiation have not been fully understood. Here, we performed lncRNA and mRNA expression profile analysis using microarray technology and identified 1064 lncRNAs with differential expression (fold change| ≥4, p ≤ 0.01) on day 0 and day 8 during differentiation. Furthermore, candidate lncRNAs were characterized by comprehensive examination of their genomic context, gene ontology (GO) enrichment of their associated protein-coding genes and pathway analysis. We identified three lncRNAs (Gm15051, Tmem189 and Cebpd) associated with their flanking coding genes (Hoxa1, C/EBPβ and C/EBPδ), which participated in adipose commitment. Collectively, our findings indicated lncRNAs are involved in mouse BAT development and provide potential targets for obesity therapy.

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Citations

Mar 16, 2016·Molecular Metabolism·Zheng Chen
Mar 5, 2016·Cellular and Molecular Life Sciences : CMLS·Shengjuan WeiMichael V Dodson
Sep 28, 2015·Trends in Biochemical Sciences·Xu-Yun Zhao, Jiandie D Lin
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Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Shui-Dong FengLiang Zhang
Aug 19, 2015·Bioscience Reports·Shaohai XuLei Sun
Sep 23, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xianwei CuiXirong Guo
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Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli
Dec 29, 2020·Medicinal Research Reviews·Qirong LuMaría-Aránzazu Martínez

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