Transcriptome and metabolome analyses provide insights into root and root-released organic anion responses to phosphorus deficiency in oat

Journal of Experimental Botany
Yanliang WangJihong Liu-Clarke

Abstract

Roots and root-released organic anions play important roles in uptake of phosphorus (P), an essential macronutrient for food production. Oat, ranking sixth in the world's cereal production, contains valuable nutritional compounds and can withstand poor soil conditions. Our aim was to investigate root transcriptional and metabolic responses of oat grown under P-deficient and P-sufficient conditions. We conducted a hydroponic experiment and measured root morphology and organic anion exudation, and analysed changes in the transcriptome and metabolome. Oat roots showed enhanced citrate and malate exudation after 4 weeks of P deficiency. After 10 d of P deficiency, we identified 9371 differentially expressed transcripts with a 2-fold or greater change (P<0.05): 48 sequences predicted to be involved in organic anion biosynthesis and efflux were consistently up-regulated; 24 up-regulated transcripts in oat were also found to be up-regulated upon P starvation in rice and wheat under similar conditions. Phosphorylated metabolites (i.e. glucose-6-phosphate, myo-inositol phosphate) were reduced dramatically, while citrate and malate, some sugars and amino acids increased slightly in P-deficient oat roots. Our data are consistent with a st...Continue Reading

References

Jun 6, 1997·Science·J M de la FuenteL Herrera-Estrella
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·PR RyanDL Jones
Feb 1, 1994·Plant Physiology·J. F. JohnsonC. P. Vance
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Oliver ThimmMark Stitt
Mar 23, 2004·Physiologia Plantarum·Ayalew LigabaHideaki Matsumoto
Dec 23, 2004·Bioinformatics·Joachim KopkaDirk Steinhauser
May 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kenji MiuraPaul M Hasegawa
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Julie MissonMarie-Christine Thibaud
Aug 31, 2006·Methods in Enzymology·Alexander I SaeedJohn Quackenbush
Nov 10, 2006·Trends in Plant Science·Christian HermansNathalie Verbruggen
Apr 24, 2007·Plant Physiology·Georgina HernándezCarroll P Vance
May 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nadine StrehmelJoachim Kopka
Oct 8, 2008·Molecular Nutrition & Food Research·Julie-Anne NazareMartine Laville
Jan 27, 2009·Journal of Experimental Botany·Wei-Yi LinTzyy-Jen Chiou
Mar 10, 2009·Biotechnology Advances·Madhusmita PanigrahyN Sarla
Feb 26, 2010·Annals of Botany·Xiao Juan Yang, Patrick M Finnegan
Mar 5, 2011·Annual Review of Plant Biology·Tzyy-Jen Chiou, Shu-I Lin
May 13, 2011·Plant Physiology·William C Plaxton, Hue T Tran
Aug 30, 2011·BMC Plant Biology·Reza KhorassaniNorbert Claassen
Jul 13, 2013·BMC Genomics·Juan J Gutierrez-GonzalezDavid F Garvin
Jan 15, 2014·Journal of Integrative Plant Biology·Zhaoliang ZhangWilliam J Lucas
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ariane C KemenAnne Osbourn
Oct 1, 2014·The British Journal of Nutrition·Derek Stewart, Gordon McDougall

❮ Previous
Next ❯

Citations

Oct 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao WangWenyan Zhang
May 1, 2021·Metabolites·Isiaka Ibrahim MuhammadNurulfiza Mat Isa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.