Transcriptome and metabolome analyses revealing the potential mechanism of seed germination in Polygonatum cyrtonema.

Scientific Reports
Rong LiuYu Wu

Abstract

Polygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, to elucidate the mechanism underlying the germination of this plant in order to meet the market demand, the metabolomic and transcriptomic analyses were performed in this study. We observed that plant hormones and α-amylase activity were differentially regulated when comparing germinated and un-germinated seeds. In addition, the metabolites related to phenylpropanoid and flavonoid biosynthesis were significantly up-accumulated in germinated seeds. Hydroxycinnamoyl derivatives and organic acids were observed to be significantly decreased during germination. The results of this study suggested that compared to un-germinated seeds, germinated seeds promote flavonoid synthesis and inhibit lignin synthesis which could be beneficial to the germination of HJ seeds. Furthermore, these results suggested that starch if hydrolyzed into glucose, which could provide the necessary energy for germination. Our results may help to establish a foundation for further research investigating the regulatory networks of seed germination and may facilitate the propagation of HJ seeds.

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Donald E RichardsNicholas P Harberd
Jun 5, 2002·The Plant Cell·Ruth R FinkelsteinChristopher D Rock
Aug 15, 2006·Plant Physiology·Martín FrigerioMiguel A Blázquez
Apr 5, 2007·Nature Protocols·Jan LisecAlisdair R Fernie
Apr 22, 2008·The New Phytologist·Michael J HoldsworthWim J J Soppe
Mar 25, 2011·Journal of Experimental Botany·Karin WeitbrechtGerhard Leubner-Metzger
May 18, 2016·Plant Science : an International Journal of Experimental Plant Biology·Zhenguo MaAbir U Igamberdiev
Apr 16, 2017·Current Opinion in Plant Biology·Ruohe Yin, Roman Ulm
Mar 15, 2019·The Plant Journal : for Cell and Molecular Biology·Maharajah PonnaiahChristophe Bailly
Jul 5, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yi-Long MaZhao-Jun Wei
Aug 24, 2019·Protein Science : a Publication of the Protein Society·Minoru Kanehisa
Oct 31, 2020·Nucleic Acids Research·Minoru KanehisaMao Tanabe

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Software Mentioned

TBtools
R
Trinity
R package
Analyst
eXpress
DESeq
GraphPad Prism
Blastx
Primer

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