Transcriptome and miRNA analyses of the response to Corynespora cassiicola in cucumber

Scientific Reports
Xiangyu WangHaiyan Fan

Abstract

Cucumber (Cucumis sativus L.) target leaf spot (TLS), which is caused by the fungus Corynespora cassiicola (C. cassiicola), seriously endangers the production of cucumber. In this assay, we performed comprehensive sequencing of the transcriptome and microRNAs (miRNAs) of a resistant cucumber (Jinyou 38) during C. cassiicola inoculation using the Illumina NextSeq 500 platform. The possible genes related to the response to C. cassiicola were associated with plant hormones, transcription factors, primary metabolism, Ca2+ signaling pathways, secondary metabolism and defense genes. In total, 150 target genes of these differentially expressed miRNAs were predicted by the bioinformatic analysis. By analyzing the function of the target genes, several candidate miRNAs that may be related to the response to C. cassiicola stress were selected. We also predicted 7 novel miRNAs and predicted their target genes. Moreover, the expression patterns of the candidate genes and miRNAs were tested by quantitative real-time RT-PCR. According to the analysis, genes and miRNAs associated with secondary metabolism, particularly the phenylpropanoid biosynthesis pathway, may play a major role in the resistance to C. cassiicola stress in cucumber. These r...Continue Reading

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Citations

Oct 8, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaoxu ZhouYushi Luan
May 1, 2021·International Journal of Molecular Sciences·Renata SłomnickaGrzegorz Bartoszewski

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Datasets Mentioned

BETA
SRP117262
SRP117230

Methods Mentioned

BETA
environmental stress
environmental stresses
PCR
RNA-seq
electrophoresis

Software Mentioned

BLAST
miRbase
MIREAP
RNAfold
DESeq

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