Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

Scientific Reports
Min SunLan Wang

Abstract

Cadmium (Cd) pollution is a serious global problem, which causes irreversible toxic effects on animals. Freshwater crab, Sinopotamon henanense, is a useful environmental indicator since it is widely distributed in benthic habitats whereby it tends to accumulate Cd and other toxicants. However, its molecular responses to Cd toxicity remain unclear. In this study, we performed transcriptome sequencing and gene expression analyses of its hepatopancreas with and without Cd treatments. A total of 7.78 G clean reads were obtained from the pooled samples, and 68,648 unigenes with an average size of 622 bp were assembled, in which 5,436 were metabolism-associated and 2,728 were stimulus response-associated that include 380 immunity-related unigenes. Expression profile analysis demonstrated that most genes involved in macromolecular metabolism, oxidative phosphorylation, detoxification and anti-oxidant defense were up-regulated by Cd exposure, whereas immunity-related genes were down-regulated, except the genes involved in phagocytosis were up-regulated. The current data indicate that Cd exposure alters gene expressions in a concentration-dependent manner. Therefore, our results provide the first comprehensive S.henanense transcriptome ...Continue Reading

References

Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Feb 1, 1996·Ecotoxicology and Environmental Safety·M DeviM Fingerman
Oct 23, 1997·Genome Research·S Audic, J M Claverie
Apr 30, 1998·Scandinavian Journal of Work, Environment & Health·L Teppo
Dec 4, 2003·Mutation Research·Michael P Waalkes
Dec 21, 2005·Ecotoxicology and Environmental Safety·J C van DykJ H J van Vuren
Jul 22, 2006·Environmental Science & Technology·Gerald T AnkleyDonald Versteeg
Jan 5, 2008·Archives of Environmental Contamination and Toxicology·Na LiJian Yang
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Dec 3, 2008·Fish & Shellfish Immunology·K VijayavelH Thilagam
May 13, 2009·Toxicology and Applied Pharmacology·Bruce A Fowler
Jun 23, 2009·Genome Research·A Sorana MorrissyMarco A Marra
Dec 10, 2009·Nature Reviews. Genetics·Michael L Metzker
Apr 3, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ann CuypersKaren Smeets
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Oct 16, 2012·Environmental Toxicology·Simone Al KaddissiOlivier Simon
Jan 8, 2013·Current Opinion in Chemical Biology·Paul A McGettigan

❮ Previous
Next ❯

Citations

Dec 6, 2016·International Journal of Molecular Sciences·Chang-Chun LiYu Peng
Jun 14, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Zhi-Jian WangYao-Guang Zhang
Oct 5, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Yanfang ZhaoXiaofeng Sheng
Jun 21, 2020·Biological Trace Element Research·Lefei JiaoQicun Zhou
May 14, 2020·Journal of Environmental Health Science & Engineering·Maryam EbrahimiNima Rezaei
Oct 7, 2018·Environmental Science and Pollution Research International·Juan WangQisheng Song
Nov 17, 2020·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Xiaochuan ZhengWenbin Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
environmental stress
environmental stresses
PCR
chip
Illumina sequencing

Software Mentioned

BLAST
Primer
ESTscan
SPSS
BLAST2GO
BLASTx
MapMan
NormFinder
DEGseq
Trinity

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.