Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components

Scientific Reports
Zhao-Qun LiJin-Jie Cui

Abstract

Differences in sex pheromone component can lead to reproductive isolation. The sibling noctuid species, Helicoverpa armigera and Helicoverpa assulta, share the same two sex pheromone components, Z9-16:Ald and Z11-16:Ald, but in opposite ratios, providing an typical example of such reproductive isolation. To investigate how the ratios of the pheromone components are differently regulated in the two species, we sequenced cDNA libraries from the pheromone glands of H. armigera and H. assulta. After assembly and annotation, we identified 108 and 93 transcripts putatively involved in pheromone biosynthesis, transport, and degradation in H. armigera and H. assulta, respectively. Semi-quantitative RT-PCR, qRT-PCR, phylogenetic, and mRNA abundance analyses suggested that some of these transcripts involved in the sex pheromone biosynthesis pathways perform. Based on these results, we postulate that the regulation of desaturases, KPSE and LPAQ, might be key factor regulating the opposite component ratios in the two sibling moths. In addition, our study has yielded large-scale sequence information for further studies and can be used to identify potential targets for the bio-control of these species by disrupting their sexual communication.

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Citations

Jan 23, 2019·Journal of Economic Entomology·Luo ZongxiuChen Zongmao
Jul 8, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Xiaoyi DouRussell Jurenka
Jun 17, 2020·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ping HuYouqing Luo

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Datasets Mentioned

BETA
SRR1565435
SRR1570898

Methods Mentioned

BETA
Illumina sequencing
PCR
electrophoresis

Software Mentioned

Trinity
blastX
Beacon Designer
MEGA6
Blast2GO
ClustalX
WEGO
ClustalX2
SPSS Statistics

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