Transcriptome comparisons identify new cell markers for theca interna and granulosa cells from small and large antral ovarian follicles

PloS One
Nicholas HatzirodosRaymond J Rodgers

Abstract

In studies using isolated ovarian granulosa and thecal cells it is important to assess the degree of cross contamination. Marker genes commonly used for granulosa cells include FSHR, CYP19A1 and AMH while CYP17A1 and INSL3 are used for thecal cells. To increase the number of marker genes available we compared expression microarray data from isolated theca interna with that from granulosa cells of bovine small (n = 10 for both theca and granulosa cells; 3-5 mm) and large (n = 4 for both theca and granulosa cells, > 9 mm) antral follicles. Validation was conducted by qRT-PCR analyses. Known markers such as CYP19A1, FSHR and NR5A2 and another 11 genes (LOC404103, MGARP, GLDC, CHST8, CSN2, GPX3, SLC35G1, CA8, CLGN, FAM78A, SLC16A3) were common to the lists of the 50 most up regulated genes in granulosa cells from both follicle sizes. The expression in theca interna was more consistent than in granulosa cells between the two follicle sizes. Many genes up regulated in theca interna were common to both sizes of follicles (MGP, DCN, ASPN, ALDH1A1, COL1A2, FN1, COL3A1, OGN, APOD, COL5A2, IGF2, NID1, LHFP, ACTA2, DUSP12, ACTG2, SPARCL1, FILIP1L, EGFLAM, ADAMDEC1, HPGD, COL12A1, FBLN5, RAMP2, COL15A1, PLK2, COL6A3, LOXL1, RARRES1, FLI1, L...Continue Reading

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Jan 21, 2014·BMC Genomics·Nicholas HatzirodosRaymond J Rodgers
Jun 24, 2014·PloS One·Nicholas HatzirodosRaymond J Rodgers
Dec 30, 2014·Endocrine Reviews·Katja HummitzschRaymond J Rodgers

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Citations

Jan 28, 2016·Reproduction in Domestic Animals = Zuchthygiene·G F IlhaP B D Gonçalves
Dec 23, 2016·Data in Brief·Sarah M RomereimAndrea S Cupp
Mar 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Ahmed S A SosaMahmoud F Nawito
Mar 5, 2019·Cancer Biomarkers : Section a of Disease Markers·Juhui ChenYongtao Zhou
Dec 19, 2019·Frontiers in Endocrinology·Mohamed A Abedel-MajedAndrea S Cupp
Jul 20, 2019·Nature Communications·X FanS M Chuva de Sousa Lopes
Feb 1, 2020·Cell·Si WangGuang-Hui Liu
Jul 1, 2021·Data in Brief·Alexandria P SniderAndrea S Cupp
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

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Datasets Mentioned

BETA
GSE39589
GSE49505

Methods Mentioned

BETA
scraping
dissection
PCR

Software Mentioned

Partek Genomics Suite

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