Transcriptome display during tilapia sex determination and differentiation as revealed by RNA-Seq analysis

BMC Genomics
Wenjing TaoDeshou Wang

Abstract

The factors determining sex in teleosts are diverse. Great efforts have been made to characterize the underlying genetic network in various species. However, only seven master sex-determining genes have been identified in teleosts. While the function of a few genes involved in sex determination and differentiation has been studied, we are far from fully understanding how genes interact to coordinate in this process. To enable systematic insights into fish sexual differentiation, we generated a dynamic co-expression network from tilapia gonadal transcriptomes at 5, 20, 30, 40, 90, and 180 dah (days after hatching), plus 45 and 90 dat (days after treatment) and linked gene expression profiles to both development and sexual differentiation. Transcriptomic profiles of female and male gonads at 5 and 20 dah exhibited high similarities except for a small number of genes that were involved in sex determination, while drastic changes were observed from 90 to 180 dah, with a group of differently expressed genes which were involved in gonadal differentiation and gametogenesis. Weighted gene correlation network analysis identified changes in the expression of Borealin, Gtsf1, tesk1, Zar1, Cdn15, and Rpl that were correlated with the expre...Continue Reading

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Citations

Feb 7, 2020·Biological Reviews of the Cambridge Philosophical Society·Meghan A CastelliClare E Holleley
Aug 8, 2020·Animal Genetics·J M YáñezG M Yoshida
Feb 14, 2021·Marine Biotechnology·Z Y WanYue Gen Hua
May 1, 2021·Genes·Álvaro S RocoMónica Bullejos
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Zhongdian DongZhongduo Wang
Oct 9, 2021·Genome Biology and Evolution·Corentin DechaudMagali Naville

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Datasets Mentioned

BETA
SUB3254083

Methods Mentioned

BETA
electrophoresis

Software Mentioned

WGCNA
R
Trimmomatic
Cuffnorm
R package
Gplot
Cuffdiff
Cufflinks
Tophat

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