Transcriptome dynamics associated with resistance and susceptibility against fusarium head blight in four wheat genotypes

BMC Genomics
Youlian PanTherese Ouellet

Abstract

Fusarium head blight (FHB) of wheat in North America is caused mostly by the fungal pathogen Fusarium graminearum (Fg). Upon exposure to Fg, wheat initiates a series of cellular responses involving massive transcriptional reprogramming. In this study, we analyzed transcriptomics data of four wheat genotypes (Nyubai, Wuhan 1, HC374, and Shaw), at 2 and 4 days post inoculation (dpi) with Fg, using RNA-seq technology. A total of 37,772 differentially expressed genes (DEGs) were identified, 28,961 from wheat and 8811 from the pathogen. The susceptible genotype Shaw exhibited the highest number of host and pathogen DEGs, including 2270 DEGs associating with FHB susceptibility. Protein serine/threonine kinases and LRR-RK were associated with susceptibility at 2 dpi, while several ethylene-responsive, WRKY, Myb, bZIP and NAC-domain containing transcription factors were associated with susceptibility at 4 dpi. In the three resistant genotypes, 220 DEGs were associated with resistance. Glutathione S-transferase (GST), membrane proteins and distinct LRR-RKs were associated with FHB resistance across the three genotypes. Genes with unique, high up-regulation by Fg in Wuhan 1 were mostly transiently expressed at 2 dpi, while many defense-a...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 7, 2002·The Plant Cell·Nancy A Eckardt
Jul 23, 2003·Current Opinion in Plant Biology·Alessandra DevotoKen Shirasu
Aug 5, 2003·Genome Génome / Conseil National De Recherches Canada·Daryl J SomersMarc Savard
Jul 31, 2004·Annual Review of Phytopathology·Guihua Bai, Gregory Shaner
Dec 24, 2004·Journal of Bioinformatics and Computational Biology·Youlian PanPierre R Fobert
Aug 5, 2005·Annual Review of Phytopathology·Jane Glazebrook
Mar 15, 2006·Molecular Plant-microbe Interactions : MPMI·Ragiba MakandarJyoti Shah
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Zuyu ZhengTesfaye Mengiste
Apr 3, 2007·Nature Protocols·Glyn Jenkins, Robert Hasterok
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Apr 8, 2009·Molecular Plant-microbe Interactions : MPMI·Melvin D Bolton
Oct 8, 2009·The New Phytologist·Stephanie WalterFiona M Doohan
May 13, 2010·BMC Bioinformatics·Alain B TchagangYoulian Pan
Jun 5, 2010·Molecular Plant-microbe Interactions : MPMI·Ragiba MakandarJyoti Shah
Jul 1, 2001·Molecular Plant Pathology·A Idnurm, B J Howlett
Nov 13, 2010·The Plant Journal : for Cell and Molecular Biology·Takeo ShimizuNaoto Shibuya
Apr 9, 2011·Cell Death and Differentiation·N S CollJ L Dangl
May 11, 2011·Sub-cellular Biochemistry·Matthew T Weirauch, T R Hughes
Jul 19, 2011·The New Phytologist·Xinli SunGary J Loake
Aug 16, 2011·The Plant Cell·John W MooreSteven H Spoel
Nov 25, 2011·Molecular Plant-microbe Interactions : MPMI·Ragiba MakandarJyoti Shah
Jan 28, 2012·Journal of Experimental Botany·Saijaliisa KangasjärviGraham Noctor
Feb 10, 2012·PLoS Pathogens·Amy Huei-Yi LeeDarrell Desveaux
Feb 23, 2012·Plant Signaling & Behavior·Carole Dubreuil-Maurizi, Benoît Poinssot
Apr 3, 2012·Archives of Biochemistry and Biophysics·Kevin FrancisGiovanni Gadda
May 9, 2012·Annual Review of Cell and Developmental Biology·Corné M J PieterseSaskia C M Van Wees

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Citations

Dec 4, 2019·Molecular Plant-microbe Interactions : MPMI·Elizabeth K BrauerThérèse Ouellet
Jan 5, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhengqiang MaYang Yuan
Jun 24, 2020·Molecular Plant Pathology·Michael MentgesAna Lilia Martinez-Rocha
Jul 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Florent DelplaceDominique Roby
Nov 7, 2019·Toxins·Nora A ForoudHermann Bürstmayr
Oct 21, 2020·Scientific Reports·Jitendra KumarShahryar F Kianian
Feb 2, 2021·Frontiers in Plant Science·Kirby T NilsenMaria A Henriquez
Feb 28, 2021·Physiologia Plantarum·Basavantraya N DevannaTilak R Sharma
Feb 12, 2021·The Plant Journal : for Cell and Molecular Biology·Selma SchurackGunther Doehlemann
Apr 21, 2021·Journal of Experimental Botany·Ana K Machado WoodKostya Kanyuka
Oct 17, 2021·Applied Microbiology and Biotechnology·Juan Carlos Torres-GuzmanGloria Angelica Gonzalez-Hernandez

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Methods Mentioned

BETA
RNA-seq
feature extraction
feature
protein folding
histone acetylation
acetylate

Software Mentioned

dynamicTreeCut R package
Gene Ontology Analyzer
STAR
Ensembl
WGCNA R package
IWGSC
Ensembl Plants
DESeq2
FASTX Toolkit

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