Transcriptome-level microarray expression profiling implicates IGF-1 and Wnt signalling dysregulation in the pathogenesis of thyroid-associated orbitopathy.

Journal of Clinical Pathology
Daniel G EzraLeandro Castellano

Abstract

The pathogenesis of thyroid-associated orbitopathy (TAO) remains unclear. The aim of this study is to elucidate the gene expression profile of orbital fat from patients with active, but untreated, TAO. A case-control gene expression study was conducted using test samples of orbital fat from TAO patients and control orbital fat specimens; apart from drugs to control thyrotoxicosis, the TAO patients had received no treatment for orbital disease. cDNA expression analysis was performed using the Affymetrix GeneChip Human Genome U133 Plus 2.0 platform and validated using quantitative PCR. The highest-ranked differentially expressed genes were dominated by IGF-1 signalling genes. These include IGF-1, IGF-1 receptor binding/signalling genes, such as SOCS3 and IRS2, and downstream signalling and transcriptional regulators, such as SGK (PDK/Akt signalling) and c-JUN. Our microarray data also demonstrate dysregulation of wingless-type MMTV (Wnt) signalling gene expression, including Wnt5a, sFRPs and DKK. Altered Wnt signalling confirms previous array findings. Further investigation of the role of Wnt signalling in TAO pathogenesis is warranted. These data also provide the first evidence of dysregulation of IGF-1 pathway genes in TAO tiss...Continue Reading

References

Jun 25, 1992·The New England Journal of Medicine·L TallstedtA Taube
Jun 1, 1981·Ophthalmology·J D Trobe
Aug 29, 2000·Cellular and Molecular Life Sciences : CMLS·T E AdamsC W Ward
Nov 10, 2000·Biochemical and Biophysical Research Communications·B R DeyP Nissley
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jane PritchardTerry J Smith
Mar 22, 2002·Pflügers Archiv : European journal of physiology·N GamperF Lang
Jun 11, 2002·The Journal of Biological Chemistry·Christina N BennettOrmond A MacDougald
May 31, 2003·Journal of Cell Science·Yoshiaki Kawano, Robert Kypta
Feb 7, 2004·The Journal of Clinical Endocrinology and Metabolism·Seema KumarRebecca S Bahn
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Jun 2, 2005·The Journal of Clinical Endocrinology and Metabolism·Mikael LantzBengt Hallengren
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Raymond S DouglasTerry J Smith
Jun 17, 2008·Investigative Ophthalmology & Visual Science·Mei-Hsiu ChenLee-Ming Chuang
Sep 4, 2008·Science Signaling·Renée van AmerongenRoel Nusse
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shanli TsuiTerry J Smith
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Raymond S DouglasTerry J Smith
Nov 15, 2008·Trends in Endocrinology and Metabolism : TEM·Constantinos ChristodoulidesAntonio Vidal-Puig
Oct 6, 2009·Trends in Endocrinology and Metabolism : TEM·Robert C Baxter, Stephen M Twigg
Apr 14, 2010·Survey of Ophthalmology·Vibhavari M NaikRaymond S Douglas
Jun 2, 2010·International Journal of Molecular Medicine·Anthony V NguyenRandall F Holcombe
Apr 23, 2011·Thyroid : Official Journal of the American Thyroid Association·Tereza PlanckMikael Lantz
Jul 12, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yang XieIgnacio I Wistuba

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Citations

Nov 15, 2015·The British Journal of Ophthalmology·Jwu Jin KhongDinesh Selva
Jan 18, 2016·BMC Ophthalmology·Nicholas J D GowerAlastair K Denniston
Nov 27, 2015·Progress in Retinal and Eye Research·James T RosenbaumStephen R Planck
Mar 15, 2015·Journal of Clinical Pathology·Ruijia YanFagang Jiang
Jun 4, 2013·The Cochrane Database of Systematic Reviews·Neda Minakaran, Daniel G Ezra
Nov 28, 2018·The Cochrane Database of Systematic Reviews·Shirin Hamed AzzamDaniel G Ezra
Nov 22, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yiyun LouFan Jin
Jul 9, 2020·Thyroid Research·Michelle Ting, Daniel G Ezra
Jan 29, 2021·Investigative Ophthalmology & Visual Science·Christine L HammondCollynn F Woeller
Jan 24, 2021·The Journal of Clinical Endocrinology and Metabolism·Giovanna Rotondo DottoreMichele Marinò
Jul 2, 2021·The British Journal of Ophthalmology·Sang Joon JungSun Young Jang
Aug 22, 2021·Journal of Endocrinological Investigation·E J Neag, T J Smith

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