Transcriptome mapping of pAR060302, a blaCMY-2-positive broad-host-range IncA/C plasmid.

Applied and Environmental Microbiology
Kevin S LangTimothy J Johnson

Abstract

The multidrug resistance-encoding plasmids belonging to the IncA/C incompatibility group have recently emerged among Escherichia coli and Salmonella enterica strains in the United States. These plasmids have a unique genetic structure compared to other enterobacterial plasmid types, a broad host range, and a propensity to acquire large numbers of antimicrobial resistance genes via their accessory regions. Using E. coli strain DH5α harboring the prototype IncA/C plasmid pAR060302, we sought to define the baseline transcriptome of IncA/C plasmids under laboratory growth and in the face of selective pressure. The effects of ampicillin, florfenicol, or streptomycin exposure were compared to those on cells left untreated at logarithmic phase using Illumina platform-based RNA sequencing (RNA-Seq). Under growth in Luria-Bertani broth lacking antibiotics, much of the backbone of pAR060302 was transcriptionally inactive, including its putative transfer regions. A few plasmid backbone genes of interest were highly transcribed, including genes of a putative toxin-antitoxin system and an H-NS-like transcriptional regulator. In contrast, numerous genes within the accessory regions of pAR060302 were highly transcribed, including the resistan...Continue Reading

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Citations

Apr 23, 2013·International Journal of Antimicrobial Agents·Carmelo S del CastilloTakashi Aoki
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Carmelo S Del CastilloTakashi Aoki
Jun 17, 2015·Plasmid·Kevin S Lang, Timothy J Johnson
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Jul 25, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rym OuertaniChedly Chouchani
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Aug 3, 2018·Clinical Microbiology Reviews·Sally R PartridgeSlade O Jensen
Sep 26, 2017·Microbiology Spectrum·Alvaro San Millan, R Craig MacLean

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