Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus

International Journal of Molecular Sciences
Rui LiZhang-Ping Yang

Abstract

MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated from bovine mammary gland tissue samples from the S. aureus-induced mastitis group and controls. miRNAs were analyzed using Solexa sequencing and bioinformatics processing for the experimental group and control group. Two miRNA libraries were constructed respectively. A total of 370 known bovine miRNAs and 341 novel mi RNAs were detected for the S. aureus and 358 known bovine miRNAs and 232 novel miRNAs for control groups. A total of 77 miRNAs in the S. aureus group showed significant differences compared to the control group. GO (Gene Ontology) analysis showed these target genes were involved in the regulation of cells, binding, etc., while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in...Continue Reading

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Citations

Mar 5, 2016·BMC Genomics·Jason Ioannidis, F Xavier Donadeu
Jul 13, 2016·Scientific Reports·Vanmathy Kasimanickam, John Kastelic
Apr 12, 2019·Reproduction in Domestic Animals = Zuchthygiene·Zhi ChenZhangping Yang
Oct 31, 2019·The FEBS Journal·Yu-Chang LaiNaoki Miura
Nov 18, 2018·International Journal of Molecular Sciences·Eveline M Ibeagha-AwemuNathalie Bissonnette
Apr 23, 2016·PloS One·Ran LiEveline Mengwi Ibeagha-Awemu
Aug 8, 2020·Reproduction, Fertility, and Development·Wangsheng ZhaoJiangjiang Zhu
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Nov 10, 2020·Research in Veterinary Science·Yu-Chang LaiNaoki Miura
Apr 4, 2021·International Journal of Molecular Sciences·Duy N DoEveline M Ibeagha-Awemu
Aug 11, 2018·Journal of Agricultural and Food Chemistry·Huimin ZhangZhangping Yang
Oct 28, 2021·Tropical Animal Health and Production·Carolina Guimarães Ramos MatosinhoMaria Raquel Santos Carvalho

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Methods Mentioned

BETA
biopsy
light microscopy
PCR

Software Mentioned

Mireap
RNA hybrid

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