Transcriptome profile of macrophages from atherosclerosis-sensitive and atherosclerosis-resistant mice

Mammalian Genome : Official Journal of the International Mammalian Genome Society
Jonathan D SmithXuejun Peng

Abstract

We performed a strain intercross between two apoE-deficient mouse strains with a large difference in lesion susceptibility and measured aortic root lesion area in 98 female F(2) progeny. Total RNA was prepared from bone marrow-derived macrophages, and RNA from the five mice with the smallest and largest lesions were used for microarray gene expression profiling. Remarkably, approximately 5% of the 12,288 expressed transcripts were differentially expressed in the atherosclerosis-susceptible and atherosclerosis-resistant bone marrow-derived macrophages (unadjusted p < 0.05), thus defining the transcriptome of macrophages associated with atherosclerosis susceptibility. Using more stringent criteria of twofold or greater change and p < 0.01, 116 and 70 transcripts were overexpressed in lesion-prone and lesion-resistant bone marrow-derived macrophages, respectively. Transcription factor binding site analysis identified two promoter elements that were found more often in the genes overexpressed in the large-lesion group, and one promoter element that was found more often in the small-lesion group. The combination of this expression profiling data with the genetic method of quantitative trait locus mapping should give powerful insight...Continue Reading

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Citations

Mar 5, 2010·EMBO Molecular Medicine·Stephen A RamseyAlan Aderem
Jun 23, 2011·Chromosoma·Romain SolinhacMartine Yerle-Bouissou
Aug 29, 2012·Immunologic Research·Francisco Victorino, Scott Alper
Oct 10, 2012·Nature Reviews. Genetics·Jonathan Flint, Eleazar Eskin
Aug 8, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael E GreenbergEphraim Sehayek
Oct 21, 2014·Journal of Atherosclerosis and Thrombosis·Ji-Young LeeSang-Hak Lee
Aug 14, 2012·The Journal of Surgical Research·Roland S CronerWerner Lang
Jul 26, 2014·Metabolism: Clinical and Experimental·Jessica B AltemusEphraim Sehayek
Jan 22, 2014·Nature Communications·Michito HamadaSatoru Takahashi
Jan 20, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Yvonne DöringChristian Weber

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