Transcriptome profiling analysis for two Tibetan wild barley genotypes in responses to low nitrogen

BMC Plant Biology
Xiaoyan QuanGuoping Zhang

Abstract

Nitrogen (N) is the most common limiting factor for crop productivity worldwide. An effective approach to solve N deficiency is to develop low N (LN) tolerant crop cultivars. Tibetan annual wild barley is well-known for its wide genetic diversity and high tolerance to poor soil fertility. Up to date, no study has been done to illustrate the mechanism of LN tolerance underlying the wild barley at transcriptional level. In this study, we employed Illumina RNA-Sequencing to determine the genotypic difference in transcriptome profile using two Tibetan wild barley genotypes differing in LN tolerance (XZ149, tolerant and XZ56, sensitive). A total of 1469 differentially expressed genes (DEGs) were identified in the two genotypes at 6 h and 48 h after LN treatment. Genetic difference existed in DEGs between XZ149 and XZ56, including transporters, transcription factors (TFs), kinases, antioxidant stress and hormone signaling related genes. Meanwhile, 695 LN tolerance-associated DEGs were mainly mapped to amino acid metabolism, starch and sucrose metabolism and secondary metabolism, and involved in transporter activity, antioxidant activities, and other gene ontology (GO). XZ149 had a higher capability of N absorption and use efficiency ...Continue Reading

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Citations

Feb 9, 2017·Genome Génome / Conseil National De Recherches Canada·Quanju XiangQiang Chen
Nov 19, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jawad Munawar ShahSyed Asad Hussain Bukhari
Apr 28, 2017·Scientific Reports·Pasquale L CurciGabriella Sonnante
Aug 30, 2017·Frontiers in Plant Science·Simone Landi, Sergio Esposito
Mar 13, 2021·Journal of Experimental Botany·Antonio MauceriAntonio Lupini

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Methods Mentioned

BETA
RNA-Seq
RNA Assay
Assay
PCR

Software Mentioned

Blast2GO
Illumina
DESeq R

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