Transcriptome profiling of ontogeny in the acridid grasshopper Chorthippus biguttulus

PloS One
Emma L BerdanFrieder Mayer

Abstract

Acridid grasshoppers (Orthoptera:Acrididae) are widely used model organisms for developmental, evolutionary, and neurobiological research. Although there has been recent influx of orthopteran transcriptomic resources, many use pooled ontogenetic stages obscuring information about changes in gene expression during development. Here we developed a de novo transcriptome spanning 7 stages in the life cycle of the acridid grasshopper Chorthippus biguttulus. Samples from different stages encompassing embryonic development through adults were used for transcriptomic profiling, revealing patterns of differential gene expression that highlight processes in the different life stages. These patterns were validated with semi-quantitative RT-PCR. Embryonic development showed a strongly differentiated expression pattern compared to all of the other stages and genes upregulated in this stage were involved in signaling, cellular differentiation, and organ development. Our study is one of the first to examine gene expression during post-embryonic development in a hemimetabolous insect and we found that only the fourth and fifth instars had clusters of genes upregulated during these stages. These genes are involved in various processes ranging f...Continue Reading

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Methods Mentioned

BETA
Assay
Illumina
RNA
Chip
RNA-Seq
PCR
RNAseq

Software Mentioned

GeneTools Analysis
bowtie2
DESeq2
trinity
interproscan
Transdecoder
RSEM
BUSCO
BLASTX
Blast2GO

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