Transcriptome profiling of the intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin.

PloS One
Brenda OppertJuan Luis Jurat-Fuentes

Abstract

Bacillus thuringiensis (Bt) crystal (Cry) proteins are effective against a select number of insect pests, but improvements are needed to increase efficacy and decrease time to mortality for coleopteran pests. To gain insight into the Bt intoxication process in Coleoptera, we performed RNA-Seq on cDNA generated from the guts of Tenebrio molitor larvae that consumed either a control diet or a diet containing Cry3Aa protoxin. Approximately 134,090 and 124,287 sequence reads from the control and Cry3Aa-treated groups were assembled into 1,318 and 1,140 contigs, respectively. Enrichment analyses indicated that functions associated with mitochondrial respiration, signalling, maintenance of cell structure, membrane integrity, protein recycling/synthesis, and glycosyl hydrolases were significantly increased in Cry3Aa-treated larvae, whereas functions associated with many metabolic processes were reduced, especially glycolysis, tricarboxylic acid cycle, and fatty acid synthesis. Microarray analysis was used to evaluate temporal changes in gene expression after 6, 12 or 24 h of Cry3Aa exposure. Overall, microarray analysis indicated that transcripts related to allergens, chitin-binding proteins, glycosyl hydrolases, and tubulins were ind...Continue Reading

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Citations

Jan 10, 2013·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Vadim R VivianiDanilo T Amaral
May 30, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Brenda OppertElena N Elpidina
Oct 11, 2015·Journal of Microbiological Methods·Patrícia Canteri de SouzaRicardo Sergio Almeida
Apr 8, 2017·Ecotoxicology and Environmental Safety·Eva Vianey Alquisira-RamírezRamón Suarez-Rodríguez
Nov 15, 2018·Journal of Fungi·Patrícia Canteri de SouzaRicardo Sergio Almeida
Oct 19, 2017·Ecotoxicology·Juan WangQisheng Song
Jan 29, 2021·Microbiology and Molecular Biology Reviews : MMBR·Daniel PinosPatricia Hernández-Martínez
Mar 2, 2021·Frontiers in Microbiology·Jizhen WeiShiheng An
Apr 15, 2018·Journal of Agricultural and Food Chemistry·Yan-Jie GaoXiu-Ping Chen

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Methods Mentioned

BETA
transgenic
RNA-Seq
ubiquitination
GTPases
PCA
chip

Software Mentioned

eArray
tBLASTx
DNASTAR
Blast2GO
454 De Novo Assembler
BLASTx
Seqman
Roche GS de
CLC

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