Transcriptome profiling of the newborn mouse lung response to acute ozone exposure

Toxicological Sciences : an Official Journal of the Society of Toxicology
Kelsa GabehartAzzeddine Dakhama

Abstract

Ozone pollution is associated with adverse effects on respiratory health in adults and children but its effects on the neonatal lung remain unknown. This study was carried out to define the effect of acute ozone exposure on the neonatal lung and to profile the transcriptome response. Newborn mice were exposed to ozone or filtered air for 3h. Total RNA was isolated from lung tissues at 6 and 24h after exposure and was subjected to microarray gene expression analysis. Compared to filtered air-exposed littermates, ozone-exposed newborn mice developed a small but significant neutrophilic airway response associated with increased CXCL1 and CXCL5 expression in the lung. Transcriptome analysis indicated that 455 genes were down-regulated and 166 genes were up-regulated by at least 1.5-fold at 6h post-ozone exposure (t-test, p < .05). At 24h, 543 genes were down-regulated and 323 genes were up-regulated in the lungs of ozone-exposed, compared to filtered air-exposed, newborn mice (t-test, p < .05). After controlling for false discovery rate, 50 genes were identified as significantly down-regulated and only a few (RORC, GRP, VREB3, and CYP2B6) were up-regulated at 24h post-ozone exposure (q < .05). Gene ontology enrichment analysis reve...Continue Reading

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Citations

Sep 28, 2015·Respiratory Research·Kelsa GabehartAzzeddine Dakhama
Oct 19, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adelaide TovarSamir N P Kelada
Sep 16, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tamas JillingSadis Matalon
Apr 24, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jonathan M CiencewickiSteven R Kleeberger
Oct 8, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ishita ChoudharyYogesh Saini

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