Transcriptome profiling provides insights into molecular mechanism in Peanut semi-dwarf mutant

BMC Genomics
Fengdan GuoYanxiu Zhao

Abstract

Plant height, mainly decided by main stem height, is the major agronomic trait and closely correlated to crop yield. A number of studies had been conducted on model plants and crops to understand the molecular and genetic basis of plant height. However, little is known on the molecular mechanisms of peanut main stem height. In this study, a semi-dwarf peanut mutant was identified from 60Co γ-ray induced mutant population and designated as semi-dwarf mutant 2 (sdm2). The height of sdm2 was only 59.3% of its wild line Fenghua 1 (FH1) at the mature stage. The sdm2 has less internode number and short internode length to compare with FH1. Gene expression profiles of stem and leaf from both sdm2 and FH1 were analyzed using high throughput RNA sequencing. The differentially expressed genes (DEGs) were involved in hormone biosynthesis and signaling pathways, cell wall synthetic and metabolic pathways. BR, GA and IAA biosynthesis and signal transduction pathways were significantly enriched. The expression of several genes in BR biosynthesis and signaling were found to be significantly down-regulated in sdm2 as compared to FH1. Many transcription factors encoding genes were identified as DEGs. A large number of genes were found different...Continue Reading

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Methods Mentioned

BETA
RNA-seq
PCR

Software Mentioned

Bowtie2 ( RNA - Seq
HISAT2
BLASTX
MeV
getorf
SOAPunke
trimmomatic
DIAMOND
hmmsearch
PerlPrimer

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