Transcriptome profiling revealed novel transcriptional regulators in maize responses to Ostrinia furnacalis and jasmonic acid

PloS One
Hai WangZhihong Lang

Abstract

Chewing insects cause severe yield losses in crop production worldwide. Crop plants counteract chewing insects by transcriptionally promoting a repertoire of defense gene products that are either toxic to, or attractive to the natural enemies of, pest insects. However, the complexity of the transcriptional reprogramming in plant defense response against chewing insects is still not well understood. In this study, the genome-wide early responses in maize seedlings to Asian corn borer (ACB, Ostrinia furnacalis) and also to jasmonic acid(JA), the pivotal phytohormone controlling plant defense response against herbivory, were transcriptionally profiled by RNA-Seq. Clustering of differentially expressed genes (DEGs) along with functional enrichment analysis revealed important biological processes regulated in response to ACB infestation and/or jasmonic acid. Moreover, DEGs with distinct expression patterns were differentially enriched with diverse families of cis-elements on their promoters. Multiple inventories of differentially expressed transcription factors (DETFs) in each DEG group were also analyzed. A transient expression assay using transfected maize protoplastswas established to examine the potential roles of DETFs in maize...Continue Reading

References

Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K C ConeB Burr
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·D TremousaygueB Lescure
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y UnoK Yamaguchi-Shinozaki
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·B ShenH K Dooner
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Peña-CortésL Willmitzer
Sep 12, 2002·Annual Review of Plant Biology·André Kessler, Ian T Baldwin
May 14, 2003·The Journal of Biological Chemistry·Weining Tang, Sharyn E Perry
Oct 21, 2004·The Plant Cell·Philippe ReymondEdward E Farmer
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·Christian DelessertRudy Dolferus
Oct 14, 2005·Journal of Chemical Ecology·Mirka MacelPeter G L Klinkhamer
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christiane SchneeJörg Degenhardt
May 30, 2006·The Plant Cell·Alexandra ToFrançois Parcy
Jul 27, 2006·The New Phytologist·David LecourieuxAlain Pugin
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Honghong HuLizhong Xiong
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Zuyu ZhengTesfaye Mengiste
Jun 26, 2007·The Plant Journal : for Cell and Molecular Biology·Kazuo NakashimaKazuko Yamaguchi-Shinozaki
Sep 11, 2007·Plant Molecular Biology·Hongmei LiChuanyou Li
Mar 5, 2008·Plant Physiology·Axel Mithöfer, Wilhelm Boland
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 5, 2008·Plant Physiology·Wim GrunewaldGodelieve Gheysen
Aug 16, 2008·The Plant Journal : for Cell and Molecular Biology·M Shahid MukhtarImre E Somssich
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric A SchmelzPeter E A Teal
Apr 25, 2009·Molecules and Cells·Sun-ji LeeSoo Young Kim
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jörg DegenhardtTed C J Turlings
Nov 17, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ana ButrónM D McMullen

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
transfection
PCR
PCA

Software Mentioned

DESeq
Cufflinks
DEXSeq
Sickle
R
BLAST2GO
HTSeq
Tophat2
MEGA6
PWMEnrich

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.