Transcriptome profiling reveals differential gene expression of detoxification enzymes in Sitophilus zeamais responding to terpinen-4-ol fumigation

Pesticide Biochemistry and Physiology
Yong HuangHai-Qun Cao

Abstract

Plant essential oils with high bioactivity provide environmental friendly alternatives for synthetic pesticides. Melaleuca alternifolia essential oil and its main constituent terpinen-4-ol have high insecticidal activity to Sitophilus zeamais Motschulsky. Terpinen-4-ol may be metabolized by human and insect cytochrome P450s. However, little is known about how insects systemically respond to terpinen-4-ol. In this study, we used an RNA-seq approach to evaluate the global gene expression of S. zeamais after terpinen-4-ol fumigation. Duplicates of fumigated and control groups, for a total of four libraries, were collected for sequencing. A total of 36,117 unigenes with an average length of 1036 bp were generated in the de novo assembled transcriptome. Comparative analysis of S. zeamais libraries identified 592 differentially expressed genes (DEGs), of which 308 and 284 genes were up- and down-regulated in response to terpinen-4-ol fumigation, respectively. GO and KEGG functional analyses were performed for up and downregulated DEGs separately, showing these DEGs were enriched for terms related to catalytic activity, carbohydrate metabolism, and xenobiotics biodegradation and metabolism. DEGs encoding enzymes for detoxification wer...Continue Reading

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