Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing

Scientific Reports
Junjie WangMingzhou Song

Abstract

Tolerance of alfalfa (Medicago sativa L.) to animal grazing varies widely within the species. However, the molecular mechanisms influencing the grazing tolerant phenotype remain uncharacterized. The objective of this study was to identify genes and pathways that control grazing response in alfalfa. We analyzed whole-plant de novo transcriptomes from grazing tolerant and intolerant populations of M. sativa ssp. falcata subjected to grazing by sheep. Among the Gene Ontology terms which were identified as grazing responsive in the tolerant plants and differentially enriched between the tolerant and intolerant populations (both grazed), most were associated with the ribosome and translation-related activities, cell wall processes, and response to oxygen levels. Twenty-one grazing responsive pathways were identified that also exhibited differential expression between the tolerant and intolerant populations. These pathways were associated with secondary metabolite production, primary carbohydrate metabolic pathways, shikimate derivative dependent pathways, ribosomal subunit composition, hormone signaling, wound response, cell wall formation, and anti-oxidant defense. Sequence polymorphisms were detected among several differentially e...Continue Reading

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Datasets Mentioned

BETA
GSE50430

Methods Mentioned

BETA
RNA-seq
electrophoresis
PCR

Software Mentioned

SOAP
blastx
ESTScan
Blastall
Trinity
Chrysalis
TGI
Blast2go
Inchworm

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