Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis

BMC Genomics
Zhi-Qiang LiuYu-Guo Zheng

Abstract

Ophiocordyceps sinensis, a worm and fungus combined mixture which Hirsutella sinensis is parasitic on the caterpillar body, has been used as a traditional medicine or healthy food in China for thousands of years. H. sinensis is reported as the only correct anamorph of O. sinensis and its main active ingredients are similar to the natural O. sinensis. H. sinensis L0106, asexual strain of O. sinensis, was isolated and identified in this study. Three transcriptomes of H. sinensis at different cultivation periods (growth period 3d, pre-stable period 6d and stable period 9d) were sequenced for the first time by RNA-Seq method, and 25,511 unigenes (3d), 25,214 unigenes (6d) and 16,245 unigenes (9d) were assembled and obtained, respectively. These unigenes of the three samples were further assembled into 20,822 unigenes (All), and 62.3 percent of unigenes (All) could be annotated based on protein databases. Subsequently, the genes and enzymes involved in the biosynthesis of the active ingredients according to the sequencing and annotation results were predicted. Based on the predictions, we further investigated the interaction of different pathway networks and the corresponding enzymes. Furthermore, the differentially expressed genes ...Continue Reading

References

Apr 2, 1976·Biochimica Et Biophysica Acta·M B Lennon, R J Suhadolnik
Jul 19, 1974·Nature·J O BishopM Richardson
Oct 20, 1995·Science·V E VelculescuK W Kinzler
Apr 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R I Samuels, I C Paterson
May 1, 1996·Trends in Microbiology·J M Clarkson, A K Charnley
Feb 24, 2001·Nature·J KawaiUNKNOWN RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 11, 2003·Annual Review of Biochemistry·Douglas L Black
May 16, 2003·Mycological Research·John P ClarksonJohn M Whipps
Jun 15, 2004·Current Opinion in Structural Biology·Liana F LareauSteven E Brenner
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Hadas Ner-GaonRobert Fluhr
Dec 14, 2004·Journal of Ethnopharmacology·E J BuenzT J Motley
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Daniel J EbboleRalph Dean
Jan 20, 2005·Gene·Stefan StammHermona Soreq
Aug 20, 2005·Journal of Applied Microbiology·C-H Dong, Y-J Yao
Apr 6, 2006·EMBO Reports·Salvino D'AmicoCharles Gerday
Jul 15, 2006·Cell·Benjamin J Blencowe
Feb 13, 2008·Molecular Ecology·J Cristobal VeraJames H Marden
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Aug 16, 2008·Genomics·Olena Morozova, Marco A Marra
Sep 30, 2008·Nature Biotechnology·Marco A van den BergRoel A L Bovenberg
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Apr 8, 2009·Nature Methods·Fuchou TangM Azim Surani
Apr 18, 2009·BMC Genomics·Xing FuPhilipp Khaitovich
Sep 25, 2010·Microbial Cell Factories·Alberto AmarettiMaddalena Rossi
Aug 9, 2012·Theory in Biosciences = Theorie in Den Biowissenschaften·Günter P WagnerVincent J Lynch
May 8, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Gilles MaussionGustavo Turecki

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Citations

Aug 9, 2016·International Journal of Biological Macromolecules·Shan LinYu-Guo Zheng
Jan 16, 2018·BioMed Research International·Zhi-Qiang LiuYu-Guo Zheng

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Datasets Mentioned

BETA
KP090933

Methods Mentioned

BETA
RNA-Seq
PCR
Electrophoresis
glycosylation
chip

Software Mentioned

Blast2GO
SOAPdenovo
BLASTX
TopHat
ExtendGene
OSWP
DNAMAN
OSGP

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