Transcriptome sequencing of Crucihimalaya himalaica (Brassicaceae) reveals how Arabidopsis close relative adapt to the Qinghai-Tibet Plateau

Scientific Reports
Qin QiaoTicao Zhang

Abstract

The extreme environment of the Qinghai-Tibet Plateau (QTP) provides an ideal natural laboratory for studies on adaptive evolution. Few genome/transcriptome based studies have been conducted on how plants adapt to the environments of QTP compared to numerous studies on vertebrates. Crucihimalaya himalaica is a close relative of Arabidopsis with typical QTP distribution, and is hoped to be a new model system to study speciation and ecological adaptation in extreme environment. In this study, we de novo generated a transcriptome sequence of C. himalaica, with a total of 49,438 unigenes. Compared to five relatives, 10,487 orthogroups were shared by all six species, and 4,286 orthogroups contain putative single copy gene. Further analysis identified 487 extremely significantly positively selected genes (PSGs) in C. himalaica transcriptome. Theses PSGs were enriched in functions related to specific adaptation traits, such as response to radiation, DNA repair, nitrogen metabolism, and stabilization of membrane. These functions are responsible for the adaptation of C. himalaica to the high radiation, soil depletion and low temperature environments on QTP. Our findings indicate that C. himalaica has evolved complex strategies for adapti...Continue Reading

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Citations

Oct 24, 2018·Frontiers in Plant Science·Rozaimi RazaliSónia Negrão
Mar 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ticao ZhangLa Qiong

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Methods Mentioned

BETA
RNA-seq
Illumina sequencing

Software Mentioned

PAML
MUSCLE
codeml
cBot Cluster Generation System
KOBAS
trimAl
PAL2NAL
OrthoMCL
Trinity
RAxML

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