Transcriptome-wide analysis of filarial extract-primed human monocytes reveal changes in LPS-induced PTX3 expression levels

Scientific Reports
Benedikt C BuerfentAchim Hoerauf

Abstract

Filarial nematodes modulate immune responses in their host to enable their survival and mediate protective effects against autoimmunity and allergies. In this study, we examined the immunomodulatory capacity of extracts from the human pathogenic filaria Brugia malayi (BmA) on human monocyte responses in a transcriptome-wide manner to identify associated pathways and diseases. As previous transcriptome studies often observed quiescent responses of innate cells to filariae, the potential of BmA to alter LPS driven responses was investigated by analyzing >47.000 transcripts of monocytes from healthy male volunteers stimulated with BmA, Escherichia coli LPS or a sequential stimulation of both. In comparison to ~2200 differentially expressed genes in LPS-only stimulated monocytes, only a limited number of differentially expressed genes were identified upon BmA priming before LPS re-stimulation with only PTX3↓ reaching statistical significance after correcting for multiple testing. Nominal significant differences were reached for metallothioneins↑, MMP9↑, CXCL5/ENA-78↑, CXCL6/GCP-2↑, TNFRSF21↓, and CCL20/MIP3α↓ and were confirmed by qPCR or ELISA. Flow cytometric analysis of activation markers revealed a reduced LPS-induced expressio...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·D H Hamer
Jan 1, 1995·World Health Organization Technical Report Series
Feb 12, 1998·The New England Journal of Medicine·A D Luster
Oct 12, 2000·Nature Cell Biology·G BergersD Hanahan
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Apr 22, 2003·Infection and Immunity·Peter SchierackSusanne Hartmann
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roshanak Tolouei SemnaniThomas B Nutman
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roshanak Tolouei SemnaniThomas B Nutman
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helen S GoodridgeMargaret M Harnett
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Subash BabuThomas B Nutman
Jul 25, 2006·Infection and Immunity·Roshanak Tolouei SemnaniThomas B Nutman
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna M DittrichEckard Hamelmann
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Corinna SchnoellerSusanne Hartmann
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Nov 26, 2008·Molecular Cell·Christine EnderGunter Meister
Apr 1, 2009·Infection and Immunity·Clare M HamiltonSandra M O'Neill
Oct 28, 2009·Nature Reviews. Immunology·Ruslan Medzhitov, Tiffany Horng
Nov 20, 2009·Nucleic Acids Research·Nuno L Barbosa-MoraisSimon Tavaré
May 26, 2011·Nature Reviews. Immunology·Judith E Allen, Rick M Maizels
Oct 11, 2011·Nature Reviews. Immunology·Chao Shi, Eric G Pamer
Dec 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc P HübnerEdward Mitre
Dec 27, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Mikio KawamuraMasato Kusunoki
Feb 11, 2012·Journal of Biomedical Science·Ramakrishna U RaoGary J Weil
Apr 18, 2012·PLoS Neglected Tropical Diseases·Kathrin ArndtsAchim Hoerauf
May 12, 2012·Mediators of Inflammation·P KunesJ Krejsek

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Methods Mentioned

BETA
flow cytometry
PCR
ELISA
blood draw
FCS
Assay

Software Mentioned

Bioconductor
R
GraphPad Prism
illuminaHumanv4
limma
Ingenuity Pathway Analysis (
FACS Diva
Bioconductor package
GraphPad
FlowJo

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