Transcriptome-Wide Analysis of Hepatitis B Virus-Mediated Changes to Normal Hepatocyte Gene Expression

PLoS Pathogens
Jason LamontagneMichael J Bouchard

Abstract

Globally, a chronic hepatitis B virus (HBV) infection remains the leading cause of primary liver cancer. The mechanisms leading to the development of HBV-associated liver cancer remain incompletely understood. In part, this is because studies have been limited by the lack of effective model systems that are both readily available and mimic the cellular environment of a normal hepatocyte. Additionally, many studies have focused on single, specific factors or pathways that may be affected by HBV, without addressing cell physiology as a whole. Here, we apply RNA-seq technology to investigate transcriptome-wide, HBV-mediated changes in gene expression to identify single factors and pathways as well as networks of genes and pathways that are affected in the context of HBV replication. Importantly, these studies were conducted in an ex vivo model of cultured primary hepatocytes, allowing for the transcriptomic characterization of this model system and an investigation of early HBV-mediated effects in a biologically relevant context. We analyzed differential gene expression within the context of time-mediated gene-expression changes and show that in the context of HBV replication a number of genes and cellular pathways are altered, in...Continue Reading

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Citations

Aug 5, 2017·Antiviral Chemistry & Chemotherapy·Jesse Jr MassonClovis S Palmer
Feb 13, 2018·Scientific Reports·Sebastian EilebrechtTimo Bund
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Aug 8, 2021·Cells·Niloofar Khoshdel-RadMassoud Vosough

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Datasets Mentioned

BETA
X02496.1
EU048698.1
AY370909.2
GSE68113

Methods Mentioned

BETA
RNA-seq
cDNA library
transgenic
cDNA
PCR

Software Mentioned

bedtools
Tuxedo
iPathwayGuide
R
Bowtie2
iPathway
Sambamba
ggplot2
edgeR
FastQC

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