Transcriptome-wide analysis reveals the progress of Cordyceps militaris subculture degeneration

PloS One
Juan YinZhongzheng Gui

Abstract

The entomopathogenic mushroom Cordyceps militaris is an important medicinal and food resource owing to its various medicinal components and pharmacological effects. However, the high frequency of strain degeneration during subculture seriously restricts the large-scale production of C. militaris, and the mechanism underlying strain degeneration remains unclear. In this study, we artificially cultured C. militaris for six generations and compared changes during fruiting body growth. The transcriptome of six generations of C. militaris strains were sequenced with the Illumine Hiseq4000. The subcultured C. militaris strains degenerated beginning at the third generation, with incomplete fruiting body growth beginning at the fourth generation. Over 9,015 unigenes and 731 new genes were identified. In addition, 35,323 alternative splicing (AS) events were detected in all samples, and more AS events occurred in the second, fourth and sixth generations. Compared with the first generation, the third generation (degenerated strain) included 2,498 differentially expressed genes (DEGs) including 1,729 up-regulated and 769 down-regulated genes. This number was higher than the number of DEGs in the second (1,892 DEGs), fourth (2,006 DEGs), f...Continue Reading

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Citations

Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Syed Amir AshrafSibte Hadi
Jan 27, 2019·Archives of Microbiology·Xiangdong XinZhongzheng Gui
Aug 15, 2019·Applied Microbiology and Biotechnology·Haiwei LouRenyong Zhao
Nov 2, 2020·International Journal of Biological Macromolecules·HuiQing SunZhenYuan Zhu

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Methods Mentioned

BETA
RNA-Seq
PCR
Hi-Tech

Software Mentioned

Qubit
TrEMBL
Java Treeview
TopHat align
SPSS
cluster
BLAST
Clustalx W
Blastall
Premier

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