Transcriptome-Wide Characterization and Functional Identification of the Aquaporin Gene Family During Drought Stress in Common Vetch

DNA and Cell Biology
Xingyi WeiWenxian Liu

Abstract

Aquaporins (AQPs) are transmembrane channels that are essential for the movement of water and other small molecules between biofilms in various physiological processes in plants. In this study, based on transcriptome-wide data, we identified and described a total of 21 AQP genes in common vetch (Vicia sativa subsp. sativa), which is an economically important pasture legume worldwide. Based on phylogenetic analyses, the VsAQPs were sorted into four subfamilies, including four plasma membrane intrinsic proteins (PIPs), six tonoplast intrinsic proteins (TIPs), seven NOD26-like intrinsic proteins, and four small basic intrinsic proteins. Furthermore, chemical and physical properties of these VsAQPs, including the isoelectric point and theoretical molecular weight, were analyzed. Analyses of the AQP signature sequences and key residues indicated the substrate specificity of each VsAQP. A set of VsAQPs was selected for gene expression analysis in a number of tissues and after drought stress treatments using real-time quantitative reverse transcription/polymerase chain reaction assays. Most of the PIPs and TIPs were proposed to have critical roles in regulating the flow of water during drought stress. Heterologous expression experimen...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 7, 1998·The Plant Journal : for Cell and Molecular Biology·X SardaT Lamaze
Feb 8, 2000·American Journal of Physiology. Renal Physiology·A S Verkman, A K Mitra
Aug 7, 2002·Current Opinion in Structural Biology·Yoshinori FujiyoshiAndreas Engel
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Franka SiefritzRalf Kaldenhoff
Mar 1, 2005·Plant Physiology·Marc BotsCelestina Mariani
Jul 22, 2005·Plant & Cell Physiology·Junko SakuraiMasayoshi Maeshima
Jan 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jochen S Hub, Bert L de Groot
Apr 24, 2008·BMC Plant Biology·Jonas A H Danielson, Urban Johanson
May 1, 2008·Annual Review of Plant Biology·Christophe MaurelVéronique Santoni
Nov 26, 2010·Biotechnology Advances·Humira SonahTilak R Sharma
Jan 19, 2011·The Plant Journal : for Cell and Molecular Biology·Gerd Patrick BienertFrançois Chaumont
Feb 8, 2011·Plant Cell Reports·Michel Edmond GhanemFrancisco Pérez-Alfocea
Feb 11, 2011·Plant Molecular Biology·Runyararo Memory Hove, Mrinal Bhave
Sep 8, 2011·Plant, Cell & Environment·Rosario Vera-EstrellaOmar Pantoja
Jan 20, 2012·Annals of the New York Academy of Sciences·Yupeng WangAndrew H Paterson
Nov 13, 2013·Plant Physiology and Biochemistry : PPB·Jelli VenkateshSe Won Park
Nov 22, 2013·PloS One·Stefan ReuscherKatsuhiro Shiratake
Dec 21, 2013·Biochimica Et Biophysica Acta·Federico AbascalRafael Zardoya
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Agustín YaneffGabriela Amodeo
Aug 7, 2014·Functional & Integrative Genomics·Preetom RegonSanjib Kumar Panda
Nov 8, 2016·Frontiers in Plant Science·Hemasundar AlavilliSanjib Kumar Panda

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Software Mentioned

MEGA
HIT
mPLoc
Plant
ORF Finder
MEME
ProtParam
TBLASTn
CLUSTALW
SVM

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