Transcriptomes of human prostate cells.

BMC Genomics
Asa J OudesAlvin Y Liu

Abstract

The gene expression profiles of most human tissues have been studied by determining the transcriptome of whole tissue homogenates. Due to the solid composition of tissues it is difficult to study the transcriptomes of individual cell types that compose a tissue. To overcome the problem of heterogeneity we have developed a method to isolate individual cell types from whole tissue that are a source of RNA suitable for transcriptome profiling. Using monoclonal antibodies specific for basal (integrin beta4), luminal secretory (dipeptidyl peptidase IV), stromal fibromuscular (integrin alpha 1), and endothelial (PECAM-1) cells, respectively, we separated the cell types of the prostate with magnetic cell sorting (MACS). Gene expression of MACS-sorted cell populations was assessed with Affymetrix GeneChips. Analysis of the data provided insight into gene expression patterns at the level of individual cell populations in the prostate. In this study, we have determined the transcriptome profile of a solid tissue at the level of individual cell types. Our data will be useful for studying prostate development and cancer progression in the context of single cell populations within the organ.

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Citations

Mar 20, 2012·Cell and Tissue Research·Alvin Y LiuLawrence D True
Jun 22, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lawrence D TrueAlvin Y Liu
May 25, 2007·Nature Reviews. Cancer·Sayyed K ZaidiGary S Stein
Jul 25, 2008·Bioinformatics·Dhammika AmaratungaYung-Seop Lee
Mar 20, 2008·Current Opinion in Rheumatology·Pascale Schwab, Robert F Klein
Apr 12, 2007·BMC Urology·Laura E PascalAlvin Y Liu
Aug 6, 2009·BMC Medical Genomics·Ivan P GorlovChristopher J Logothetis
May 25, 2011·BMC Medical Genomics·Laura E PascalAlvin Y Liu
Oct 17, 2009·Journal of Strength and Conditioning Research·Gerard MorasIñigo Mujika
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Aug 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jacob Bock AxelsenEytan Domany
Feb 9, 2012·The Prostate·Eneida F VêncioAlvin Y Liu
Mar 8, 2007·The Prostate·Marc A Dall'EraAlvin Y Liu
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Dec 19, 2015·Endocrinology·Magdalena M GrabowskaRobert J Matusik
Aug 27, 2015·The Prostate·Sue-Ing QuekAlvin Y Liu
Jan 17, 2016·Journal of Cellular Physiology·Gisely T BorgesAlvin Y Liu
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Jul 23, 2020·Cell Reports·Sajjan KoiralaPatrick Ryan Potts
Dec 20, 2018·Cell Reports·Gervaise H HenryDouglas W Strand

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Methods Mentioned

BETA
flow cytometry
fluorescence-activated cell sorting
FACS
in vitro transcription
chips
chip

Software Mentioned

Softmatrix
Photoshop CS
GeneAtlas
GeneSpring
FACS
GeneSpring RMA
Affymetrix
GeneChip Operating Software
Bioconductor

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