Transcriptomic analyses of xylan degradation by Prevotella bryantii and insights into energy acquisition by xylanolytic bacteroidetes.

The Journal of Biological Chemistry
Dylan DoddIsaac K O Cann

Abstract

Enzymatic depolymerization of lignocellulose by microbes in the bovine rumen and the human colon is critical to gut health and function within the host. Prevotella bryantii B(1)4 is a rumen bacterium that efficiently degrades soluble xylan. To identify the genes harnessed by this bacterium to degrade xylan, the transcriptomes of P. bryantii cultured on either wheat arabinoxylan or a mixture of its monosaccharide components were compared by DNA microarray and RNA sequencing approaches. The most highly induced genes formed a cluster that contained putative outer membrane proteins analogous to the starch utilization system identified in the prominent human gut symbiont Bacteroides thetaiotaomicron. The arrangement of genes in the cluster was highly conserved in other xylanolytic Bacteroidetes, suggesting that the mechanism employed by xylan utilizers in this phylum is conserved. A number of genes encoding proteins with unassigned function were also induced on wheat arabinoxylan. Among these proteins, a hypothetical protein with low similarity to glycoside hydrolases was shown to possess endoxylanase activity and subsequently assigned to glycoside hydrolase family 5. The enzyme was designated PbXyn5A. Two of the most similar protei...Continue Reading

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Citations

Jun 14, 2013·Animal : an International Journal of Animal Bioscience·S C LeahyG T Attwood
Jan 17, 2012·Applied and Environmental Microbiology·Xiaoyun SuIsaac K O Cann
Sep 21, 2012·BMC Evolutionary Biology·Henrik AspeborgBernard Henrissat
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Jan 12, 2011·Molecular Microbiology·Dylan DoddIsaac K O Cann
Jul 25, 2019·Applied Microbiology and Biotechnology·Ravindra Pal Singh

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