Transcriptomic analysis comparing stay-green and senescent Sorghum bicolor lines identifies a role for proline biosynthesis in the stay-green trait

Journal of Experimental Botany
Stephanie M JohnsonMarc R Knight

Abstract

Sorghum bicolor is an important cereal crop grown on the arid and semi-arid regions of >98 different countries. These regions are such that this crop is often subjected to low water conditions, which can compromise yields. Stay-green sorghum plants are able to retain green leaf area for longer under drought conditions and as such have higher yields than their senescent counterparts. However, the molecular and physiological basis of this drought tolerance is yet to be fully understood. Here, a transcriptomic approach was used to compare gene expression between stay-green (B35) and senescent (R16) sorghum varieties. Ontological analysis of the differentially expressed transcripts identified an enrichment of genes involved with the 'response to osmotic stress' Gene Ontology (GO) category. In particular, delta1-pyrroline-5-carboxylate synthase 2 (P5CS2) was highly expressed in the stay-green line compared with the senescent line, and this high expression was correlated with higher proline levels. Comparisons of the differentially expressed genes with those that lie in known stay-green qualitative trait loci (QTLs) revealed that P5CS2 lies within the Stg1 QTL. Polymorphisms in known cis-elements were identified in the putative promo...Continue Reading

References

Apr 1, 1996·Plant Physiology·A J WoodP B Goldsbrough
Aug 4, 1999·Trends in Plant Science·O J Goddijn, van Dun K
Jul 21, 2000·Genome Génome / Conseil National De Recherches Canada·W XuH T Nguyen
Aug 12, 2000·Journal of Experimental Botany·H Thomas, C J Howarth
Feb 13, 2001·Current Opinion in Plant Biology·N Dengler, J Kang
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 10, 2002·Plant Molecular Biology·A C SanchezH T Nguyen
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Sean D SimpsonKazuko Yamaguchi-Shinozaki
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B I G HaussmannH H Geiger
Mar 21, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y-G ShenS-Y Chen
Jan 1, 2004·Plant Physiology·Keyan Zhu-SalzmanHisashi Koiwa
May 3, 2005·Annual Review of Plant Biology·Paula McSteen, Ottoline Leyser
Sep 15, 2005·Plant Molecular Biology·Christina D BuchananJohn E Mullet
Nov 24, 2006·Journal of Experimental Botany·John Passioura
Dec 19, 2006·Journal of Experimental Botany·Karen HarrisJohn Mullet
Oct 29, 1982·Science·J S Boyer
Aug 8, 2008·Bioscience, Biotechnology, and Biochemistry·Yi-yue ZhangQi Xie
Feb 4, 2009·Nature·Andrew H PatersonDaniel S Rokhsar
May 4, 2010·Nucleic Acids Research·Zhou DuZhen Su
Sep 23, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E S MaceD R Jordan
Oct 1, 2011·Plant Science : an International Journal of Experimental Plant Biology·Man SuGong-She Liu
Oct 14, 2011·Nature·Jonathan A FoleyDavid P M Zaks
Feb 2, 2013·Plant Physiology·Dong ZhangAndrew H Paterson
Mar 7, 2014·Journal of Experimental Botany·Howard Thomas, Helen Ougham
Jun 12, 2014·BMC Genomics·Stephanie M JohnsonMarc R Knight
Mar 1, 2003·Functional Plant Biology : FPB·Manuela M ChavesJoão S Pereira

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Citations

Nov 24, 2019·International Journal of Molecular Sciences·Nasrein Mohamed KamalHisashi Tsujimoto
Sep 14, 2018·Frontiers in Plant Science·Sangam L DwivediRodomiro Ortiz
Dec 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nelle VaroquauxElizabeth Purdom
Nov 17, 2020·Frontiers in Genetics·Min Tu, Yin Li

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Datasets Mentioned

BETA
GPL17335

Methods Mentioned

BETA
Feature Extraction
PCR
chips
chip

Software Mentioned

GeneSpring GX
AgriGO
ClustalW2
MAS
Agilent Feature Extraction
GeneSpring
Fluidigm Real - Time PCR

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