Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Cell Transplantation
Ricardo F FraustoAnthony J Aldave

Abstract

The corneal endothelium plays a primary role in maintaining corneal homeostasis and clarity and must be surgically replaced with allogenic donor corneal endothelium in the event of visually significant dysfunction. However, a worldwide shortage of donor corneal tissue has led to a search for alternative sources of transplantable tissue. Cultured human corneal endothelial cells (HCEnC) have been shown to restore corneal clarity in experimental models of corneal endothelial dysfunction in animal models, but characterization of cultured HCEnC remains incomplete. To this end, we utilized next-generation RNA sequencing technology to compare the transcriptomic profile of ex vivo human corneal endothelial cells (evHCEnC) with that of primary HCEnC (pHCEnC) and HCEnC lines and to determine the utility of cultured and immortalized corneal endothelial cells as models of in vivo corneal endothelium. Multidimensional analyses of the transcriptome data sets demonstrated that primary HCEnC have a closer relationship to evHCEnC than do immortalized HCEnC. Subsequent analyses showed that the majority of the genes specifically expressed in HCEnC (not expressed in ex vivo corneal epithelium or fibroblasts) demonstrated a marked variability of ex...Continue Reading

References

May 1, 1987·Experimental Eye Research·R W YeeM E Stern
Aug 1, 1967·Archives of Ophthalmology·J E Harris
Dec 11, 1999·Science·M GriffithM A Watsky
Nov 16, 2001·Archives of Ophthalmology·A S JunJ D Gottsch
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Shaun P Collin, H Barry Collin
Jan 31, 2003·Investigative Ophthalmology & Visual Science·John D GottschSammy H Liu
Feb 14, 2003·Archives of Ophthalmology·John D GottschSammy H Liu
Feb 25, 2003·Progress in Retinal and Eye Research·Joseph A Bonanno
Dec 5, 2003·Nucleic Acids Research·Xiaowei Wang, Brian Seed
Jul 24, 2007·Nature Reviews. Molecular Cell Biology·Thomas Lecuit, Pierre-François Lenne
May 7, 2009·Current Opinion in Ophthalmology·Isabel DapenaGerrit R J Melles
Jul 16, 2010·Investigative Ophthalmology & Visual Science·Rita GruschwitzKatrin Engelmann
Apr 24, 2012·Acta Biomaterialia·Benjamin Kim Kiat TeoEvelyn King Fai Yim
May 15, 2013·Clinical Genetics·A J AldaveR F Frausto
Jun 8, 2013·Investigative Ophthalmology & Visual Science·Yuen Kuen CheongWilliam Sun
Jul 3, 2013·Trends in Genetics : TIG·Eli Eisenberg, Erez Y Levanon
Nov 26, 2013·Cell Transplantation·Gary S L PehJodhbir S Mehta
Dec 10, 2013·Bioinformatics·Yuwen LiuKevin P White
Jul 19, 2014·Investigative Ophthalmology & Visual Science·Eunbi KimYoung Joo Shin
Nov 8, 2014·Investigative Ophthalmology & Visual Science·Ricardo F FraustoAnthony J Aldave
Dec 23, 2014·Cellular and Molecular Bioengineering·Rachelle N PalcheskoAdam W Feinberg

❮ Previous
Next ❯

Citations

Dec 30, 2016·Clinical & Experimental Ophthalmology·Richárd NagymihályGoran Petrovski
Feb 13, 2018·Journal of Tissue Engineering and Regenerative Medicine·Bert Van den BogerdNadia Zakaria
Jul 26, 2019·Investigative Ophthalmology & Visual Science·Vinay Swamy, David McGaughey
Jul 29, 2020·Investigative Ophthalmology & Visual Science·Wenlin ZhangAnthony J Aldave
Aug 1, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David EvelethRalph A Bradshaw
Dec 28, 2017·Current Eye Research·Magnus KruseMatthias Fuest
May 31, 2018·Investigative Ophthalmology & Visual Science·Muhammad AliS Amer Riazuddin
Mar 27, 2019·Therapeutic Advances in Ophthalmology·Nicholas Owen, Mariya Moosajee
Dec 8, 2019·Current Eye Research·Naoki Okumura, Noriko Koizumi
May 6, 2020·Scientific Reports·Ricardo F FraustoAnthony J Aldave
Sep 2, 2016·American Journal of Physiology. Cell Physiology·Liyo KaoIra Kurtz
Sep 7, 2019·Experimental Eye Research·Susumu HaraKohji Nishida
Dec 29, 2020·Acta Ophthalmologica·Daniele SpinozziSilke Oellerich
Feb 17, 2021·Development·Natalie J Milmoe, Abigail S Tucker
Apr 4, 2021·Experimental Eye Research·Silvia Rodríguez-FernándezSilvia M Díaz-Prado
Apr 22, 2021·Tissue Engineering. Part B, Reviews·Joris Van MeenenCarina Koppen
Jul 9, 2021·Progress in Retinal and Eye Research·Pere CatalàMor M Dickman
Aug 13, 2021·Scientific Reports·Ann J LigockiCarmelo Romano

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE65991

Methods Mentioned

BETA
Electrophoresis
Hi-seq
RNA-seq
PCA
PCR

Software Mentioned

GraphPad Prism
evHCEnC
Partek Genomics Suite
Ensemble
TopHat aligner
HCEnC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Investigative Ophthalmology & Visual Science
Ricardo F FraustoAnthony J Aldave
The Journal of Nursing Administration
K C Kelly
Ophthalmology
J P McCulleyB D Schwartz
© 2021 Meta ULC. All rights reserved