Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture

Histochemistry and Cell Biology
Magdalena KulusPaweł Antosik

Abstract

The primary function of ovarian granulosa cells (GCs) is the support of oocytes during maturation and development. Molecular analyses of granulosa cell-associated processes, leading to improvement of understanding of the cell cycle events during the formation of ovarian follicles (folliculogenesis), may be key to improve the in vitro fertilization procedures. Primary in vitro culture of porcine GCs was employed to examine the changes in the transcriptomic profile of genes belonging to "cell cycle", "cell division", "cell cycle process", "cell cycle phase transition", "cell cycle G1/S phase transition", "cell cycle G2/M phase transition" and "cell cycle checkpoint" ontology groups. During the analysis, microarrays were employed to study the transcriptome of GCs, analyzing the total RNA of cells from specific periods of in vitro cultures. This research was based on material obtained from 40 landrace gilts of similar weight, age and the same living conditions. RNA was isolated at specific timeframes: before the culture was established (0 h) and after 48 h, 96 h and 144 h in vitro. Out of 133 differentially expressed genes, we chose the 10 most up-regulated (SFRP2, PDPN, PDE3A, FGFR2, PLK2, THBS1, ETS1, LIF, ANXA1, TGFB1) and the 1...Continue Reading

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Citations

Jun 6, 2020·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Apr 23, 2021·Endocrine Regulations·Alzbeta Bujnakova Mlynarcikova, Sona Scsukova

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Datasets Mentioned

BETA
GSE134361

Methods Mentioned

BETA
transcriptomic profiling
chips
PCR

Software Mentioned

Affymetrix [UNK]
REACTOME FIViz
STRING
Bioconductor
R
DAVID
DAVID ( Database for Annotation , Visualization and Integrated...
ReactomeFIViz
GeneAtlas
STRING10 ( Search Tool for the Retrieval of Interacting Genes )

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