Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans.

BMC Plant Biology
Jianxin FuHongbo Zhao

Abstract

Sweet osmanthus (Osmanthus fragrans Lour.) is one of the top ten traditional ornamental flowers in China. The flowering time of once-flowering cultivars in O. fragrans is greatly affected by the relatively low temperature, but there are few reports on its molecular mechanism to date. A hypothesis had been raised that genes related with flower opening might be up-regulated in response to relatively low temperature in O. fragrans. Thus, our work was aimed to explore the underlying molecular mechanism of flower opening regulated by relatively low temperature in O. fragrans. The cell size of adaxial and abaxial petal epidermal cells and ultrastructural morphology of petal cells at different developmental stages were observed. The cell size of adaxial and abaxial petal epidermal cells increased gradually with the process of flower opening. Then the transcriptomic sequencing was employed to analyze the differentially expressed genes (DEGs) under different number of days' treatments with relatively low temperatures (19 °C) or 23 °C. Analysis of DEGs in Gene Ontology analysis showed that "metabolic process", "cellular process", "binding", "catalytic activity", "cell", "cell part", "membrane", "membrane part", "single-organism process",...Continue Reading

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Methods Mentioned

BETA
genetic modification
transmission electron microscopy
RNA-seq
phosphotransfer
Illumina sequencing
PCR

Software Mentioned

Blastn
Blastx
Diamond
InterProScan5
OfACT
Blast2GO
SPSS
Image J

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