Transcriptomic Analysis of Fuzi Lizhong Decoction for the Treatment of Stomach Ulcers

Evidence-based Complementary and Alternative Medicine : ECAM
Yang XinLei Xu

Abstract

This study aims to understand the treatment of stomach ulcers with FLD and to identify its potential target genes as well as related pathways by transcriptomic analysis. Rat stomach mRNA from a blank group (BG), a model group (MG), an untreated-model group (u-MG), and a treated group (TG) was sequenced. A partial least-squares discriminant analysis (PLS-DA) was used to differentiate the MG from the BG, and the Deseq2 R Package was used to identify differentially expressed genes between these groups. Furthermore, t-tests based on transcripts per million (TPM) were used to select different genes between MG and BG and significantly retrieved genes in TG, except for self-retrieved genes in u-MG. Finally, pathways regulated by retrieved genes were analyzed with KEGG database. Results showed that 327 of the 32,623 total detected genes were different (p < 0.05) between the MG and BG. Among these genes, eighteen genes were significantly retrieved after rats were treated with FLD in TG, and they were considered as target genes on which FLD acted. In conclusion, FLD was deduced to cure stomach ulcers by affecting glycerolipid metabolism, fatty acid degradation, circadian entrainment, circadian rhythm, and dopaminergic synapse.

References

Jan 1, 1992·American Journal of Surgery·J G MooreM T Dayton
May 28, 1985·European Journal of Pharmacology·P SikirićA Marović
Sep 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G OnerU K Sentürk
Oct 1, 2009·Chemico-biological Interactions·Jong-Il ChoiJu-Woon Lee
Aug 19, 2010·Journal of Lipid Research·Jeffrey D Browning, Jay D Horton
Nov 6, 2012·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Shintaro FujiharaTsutomu Masaki
Jan 2, 2013·Journal of Ethnopharmacology·M S BrandãoF R C Almeida
Mar 27, 2018·Trends in Genetics : TIG·Jan Verheijen, Kristel Sleegers
May 5, 2018·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bai Xue-QinLiu Lu-Shan
Jun 12, 2019·Neurobiology of Learning and Memory·Michael R DugganVinay Parikh
May 1, 2018·Asian Journal of Pharmaceutical Sciences·Qian LiYuyang Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
electrophoresis
bioelectrophoresis

Software Mentioned

DESeq2
Trimmomatic
SnpEff
db
SPSS Statistics
GffCompare
ASprofier
HISAT2
R igraph package
BEDTools

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.