Transcriptomic Analysis of Intestinal Tissues from Two 90-Day Feeding Studies in Rats Using Genetically Modified MON810 Maize Varieties

Frontiers in Genetics
Jutta SharbatiRalf Einspanier

Abstract

Background: Global as well as specific expression profiles of selected rat tissues were characterized to assess the safety of genetically modified (GM) maize MON810 containing the insecticidal protein Cry1Ab. Gene expression was evaluated by use of Next Generation Sequencing (NGS) as well as RT-qPCR within rat intestinal tissues based on mandatory 90-day rodent feeding studies. In parallel to two 90-day feeding studies, the transcriptional response of rat tissues was assessed as another endpoint to enhance the mechanistic interpretation of GM feeding studies and/or to facilitate the generation of a targeted hypothesis. Rats received diets containing 33% GM maize (MON810) or near-isogenic control maize. As a site of massive exposure to ingested feed the transcriptomic response of ileal and colonic tissue was profiled via RT-qPCR arrays targeting apoptosis, DNA-damage/repair, unfolded protein response (UPR). For global RNA profiling of rat ileal tissue, we applied NGS. Results: No biological response to the GM-diet was observed in male and in female rat tissues. Transcriptome wide analysis of gene expression by RNA-seq confirmed these findings. Nevertheless, gene ontology (GO) analysis clearly associated a set of distinctly regul...Continue Reading

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Citations

Dec 12, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xavier CoumoulBernard Salles
Jul 7, 2020·EFSA Journal·Anna LanzoniMaged Younes
Jun 26, 2018·Frontiers in Genetics·Sami Dridi

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Datasets Mentioned

BETA
PRJNA400118
GM

Methods Mentioned

BETA
transgenic
PCR
RNAseq
RNA-seq

Software Mentioned

STRING
Tophat
CummeRbund
Excel
Cytoscape
Cuffdiff
SPSS Statistics
RefFinder
Bio1D
Cufflinks

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis