Transcriptomic analysis of porcine PBMCs in response to Actinobacillus pleuropneumoniae reveals the dynamic changes of differentially expressed genes related to immuno-inflammatory responses

Antonie van Leeuwenhoek
Hexiang JiangLiancheng Lei

Abstract

Actinobacillus pleuropneumoniae is the cause of porcine pleuropneumonia, for which the mortality rate is high. Host peripheral blood is a body site for the immune clearance of pathogens mediated by release of inflammatory factors. However, "out of control" inflammatory factor release can contribute to host death. To further understand the changes in the transcription level of immune-related effectors, samples of peripheral blood mononuclear cells (PBMCs) collected from piglets at different stages of infection (0, 24 and 120 h) were sequenced on an Illumina HiSeq™ 4000 platform. We found 3818 differentially expressed genes (DEGs) in the 24 h-infection group compared to the 0 h-infection group (Pb24-Vs-Pb0). DEGs mainly involved in the Gene ontology and KEGG pathways that included nucleic acid metabolism regulation, cell growth, cell differentiation, and organ morphological maintenance were not significantly enriched (P > 0.05). However, DEGs associated with protein kinase activity, receptor activation, metabolism, local adhesion and immune inflammatory responses were significantly enriched in Pb120-Vs-Pb24 (P < 0.05), as were those related to the T cell receptor signalling pathway, with most being down-regulated compared to the ...Continue Reading

References

Mar 7, 2001·Cellular and Molecular Life Sciences : CMLS·U Schönbeck, P Libby
Nov 10, 2001·Nature Immunology·R S BystryA G Betz
Apr 27, 2002·The Journal of Biological Chemistry·Fabio Re, Jack L Strominger
Oct 22, 2003·Circulation·Paul E SzmitkoSubodh Verma
May 20, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dirk FoellJohannes Roth
Jun 19, 2004·The International Journal of Biochemistry & Cell Biology·M Maurer, E von Stebut
Nov 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Atsushi KosakiToshiji Iwasaka
Apr 14, 2006·Veterinary Research·Eileen L Thacker
Oct 31, 2006·Oncogene·M S HaydenS Ghosh
Jan 9, 2007·The Journal of Allergy and Clinical Immunology·Zheng YangCarolyn L Geczy
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Oct 31, 2009·Circulation Research·Marion Hofmann BowmanElizabeth M McNally
Apr 1, 2011·Mucosal Immunology·P S RedfordA O'Garra
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jennifer R TisoncikMichael G Katze
Dec 20, 2013·International Journal of Molecular Sciences·Shumin YuYanchun Hu
Jan 22, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Gerald ReinerKarl Heinz Waldmann
Aug 12, 2014·International Journal of Molecular Sciences·Xinyun LiShuhong Zhao
Aug 15, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Gerald ReinerRalf Herwig
Dec 9, 2014·The International Journal of Biochemistry & Cell Biology·C K HindM S Cragg
Mar 10, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel M ReedJane A Mitchell
Jul 8, 2015·Proteomics·Alberto Benito-Martin, Héctor Peinado
Oct 19, 2016·European Journal of Immunology·Jennifer L Gardell, David C Parker

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Methods Mentioned

BETA
ELISA
X-ray
lavage
RNA-Seq

Software Mentioned

FunRich
SOAPaligner
Blast2GO
Cytoscape ClueGo
PrimerQuest
SOAP2
DAVID Bioinformatics Resources
BLAST

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