Transcriptomic analysis of salt stress responsive genes in Rhazya stricta

PloS One
Nahid H HajrahRobert K Jansen

Abstract

Rhazya stricta is an evergreen shrub that is widely distributed across Western and South Asia, and like many other members of the Apocynaceae produces monoterpene indole alkaloids that have anti-cancer properties. This species is adapted to very harsh desert conditions making it an excellent system for studying tolerance to high temperatures and salinity. RNA-Seq analysis was performed on R. stricta exposed to severe salt stress (500 mM NaCl) across four time intervals (0, 2, 12 and 24 h) to examine mechanisms of salt tolerance. A large number of transcripts including genes encoding tetrapyrroles and pentatricopeptide repeat (PPR) proteins were regulated only after 12 h of stress of seedlings grown in controlled greenhouse conditions. Mechanisms of salt tolerance in R. stricta may involve the upregulation of genes encoding chaperone protein Dnaj6, UDP-glucosyl transferase 85a2, protein transparent testa 12 and respiratory burst oxidase homolog protein b. Many of the highly-expressed genes act on protecting protein folding during salt stress and the production of flavonoids, key secondary metabolites in stress tolerance. Other regulated genes encode enzymes in the porphyrin and chlorophyll metabolic pathway with important roles ...Continue Reading

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Citations

Jul 26, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sanjay GairolaKareem A Mosa
May 3, 2020·Molecular Biology Reports·Ahmed M Ramadan
Sep 24, 2018·Molecular Biotechnology·Wei Tang, Caroline Luo

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Methods Mentioned

BETA
salt stress
RNA-Seq
protein folding
PCR
environmental stress
environmental stresses
salt

Software Mentioned

R
Netprimer
Blastx
EdgeR
Bowtie
Trinity RNA - Seq Assembly package
RSEM
blast2GO
blast
- GO

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