Transcriptomic Analysis of Tea Plant Responding to Drought Stress and Recovery

PloS One
Sheng-Chuan LiuLiang Chen

Abstract

Tea plant (Camellia sinensis) is an economically important beverage crop. Drought stress (DS) seriously limits the growth and development of tea plant, thus affecting crop yield and quality. To elucidate the molecular mechanisms of tea plant responding to DS, we performed transcriptomic analysis of tea plant during the three stages [control (CK) and during DS, and recovery (RC) after DS] using RNA sequencing (RNA-Seq). Totally 378.08 million high-quality trimmed reads were obtained and assembled into 59,674 unigenes, which were extensively annotated. There were 5,955 differentially expressed genes (DEGs) among the three stages. Among them, 3,948 and 1,673 DEGs were up-regulated under DS and RC, respectively. RNA-Seq data were further confirmed by qRT-PCR analysis. Genes involved in abscisic acid (ABA), ethylene, and jasmonic acid biosynthesis and signaling were generally up-regulated under DS and down-regulated during RC. Tea plant potentially used an exchange pathway for biosynthesis of indole-3-acetic acid (IAA) and salicylic acid under DS. IAA signaling was possibly decreased under DS but increased after RC. Genes encoding enzymes involved in cytokinin synthesis were up-regulated under DS, but down-regulated during RC. It se...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 9, 2003·Journal of Plant Physiology·Claudio StasollaHiroshi Ashihara
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Sep 16, 2006·The Plant Journal : for Cell and Molecular Biology·Pablo A ManavellaRaquel L Chan
Jan 26, 2008·The Journal of Biological Chemistry·Yoko OgasawaraKazuyuki Kuchitsu
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Mar 24, 2009·Cell·Dolf Weijers, Jirí Friml
Oct 27, 2009·Nature·Ken-Ichi MiyazonoMasaru Tanokura
Dec 29, 2009·Trends in Plant Science·László Szabados, Arnould Savouré
Mar 3, 2010·Annual Review of Plant Biology·Tae-Houn KimJulian I Schroeder
Jul 16, 2010·The Plant Journal : for Cell and Molecular Biology·Stefan MeyerRainer Hedrich
Oct 29, 2010·Plant & Cell Physiology·Taishi UmezawaKazuko Yamaguchi-Shinozaki
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
May 4, 2011·Journal of Integrative Plant Biology·Chuanfu An, Zhonglin Mou
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
May 17, 2011·Molecular Biology Reports·Guo-Tao HuangZhi-Fu Guo
Sep 8, 2011·Nature Reviews. Genetics·Jeffrey A Martin, Zhong Wang
Jan 13, 2012·Trends in Plant Science·Sukbong HaLam-Son Phan Tran
Mar 1, 2012·Molecular Biotechnology·Sushmita GuptaSudripta Das
Mar 27, 2012·Journal of Experimental Botany·Yoshihiro Mano, Keiichirou Nemoto
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·Yuge LiJinfa Wang
May 5, 2012·Annual Review of Plant Biology·Ildoo HwangBruno Müller
May 9, 2012·Functional & Integrative Genomics·Sushmita GuptaSudripta Das
Sep 6, 2012·Plant, Cell & Environment·Niranjani J IyerRamamurthy Mahalingam
Nov 13, 2012·Molecular Plant·Kemal Kazan, John M Manners
May 30, 2013·Frontiers in Plant Science·Agata Daszkowska-Golec, Iwona Szarejko
Aug 1, 2013·Nature Communications·Kenji KomatsuYoichi Sakata
Dec 3, 2013·Journal of Experimental Botany·David GranotRakefet David-Schwartz
Mar 4, 2014·Annual Review of Plant Biology·Gemma FarréChangfu Zhu
Jun 12, 2014·BMC Genomics·Stephanie M JohnsonMarc R Knight
Jul 30, 2014·Current Opinion in Plant Biology·Susheng SongDaoxin Xie

❮ Previous
Next ❯

Citations

Nov 27, 2019·Journal of Experimental Botany·Maximiliano Estravis-BarcalaMaría Verónica Arana
Nov 22, 2016·Frontiers in Plant Science·Muthusamy MuthusamyArumugam Chandrasekar
Jan 8, 2020·Horticulture Research·En-Hua XiaXiao-Chun Wan
Jan 1, 2021·Horticulture Research·En-Hua XiaXiao-Chun Wan
May 26, 2021·Journal of the Science of Food and Agriculture·Zhidong LvChengwen Shen

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA297732

Methods Mentioned

BETA
RNA-Seq
electrophoresis
PCR
ubiquitination
transgenic

Software Mentioned

BLASTx
ESTScan
Blast2GO
BLAST
TGICL
Trinity
WEGO
BINGO
Cluster
Expectation - Maximization ( RSEM )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.