Transcriptomic analysis of the underground renewal buds during dormancy transition and release in 'Hangbaishao' peony (Paeonia lactiflora)

PloS One
Jiaping ZhangYiping Xia

Abstract

Paeonia lactiflora is one of the most famous species of herbaceous peonies with gorgeous flowers. Bud dormancy is a crucial developmental process that allows P. lactiflora to survive unfavorable environmental conditions. However, little information is available on the molecular mechanism of the bud dormancy in P. lactiflora. We performed de novo transcriptome sequencing using the Illumina RNA sequencing platform for the underground renewal buds of P. lactiflora 'Hangbaishao' to study the molecular mechanism underlying its bud dormancy transition (the period from endodormancy to ecodormancy) and release (the period from ecodormancy to bud elongation and sprouting). Approximately 300 million high-quality clean reads were generated and assembled into 207,827 (mean length = 828 bp) and 51,481 (mean length = 1250 bp) unigenes using two assembly methods named "Trinity" and "Trinity+PRICE", respectively. Based on the data obtained by the latter method, 32,316 unigenes were annotated by BLAST against various databases. Approximately 1,251 putative transcription factors were obtained, of which the largest number of unique transcripts belonged to the basic helix-loop-helix protein (bHLH) transcription factor family, and five of the top t...Continue Reading

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Citations

Mar 24, 2018·International Journal of Molecular Sciences·Yuanzheng YueHuirong Hu
Dec 1, 2019·Planta·Yongming FanXiaonan Yu

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Datasets Mentioned

BETA
PRJNA245064
SRR1269650
GBFN00000000

Methods Mentioned

BETA
chemical treatment
cDNA library construction
PCR
enzyme-linked immunosorbent assay
RNA-Seq
environmental stress
transgenic
environmental stresses
histone acetylation

Software Mentioned

FASTX
CAP3
KEGG Automatic Annotation Server ( KAAS )
- Toolkit
SOAPdenovo
- Iterative Contig Extension ( PRICE )
MISA
Basic Local Alignment Search Tool ( BLAST )
Blast2GO
BLAST

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