Transcriptomic and computational analysis identified LPA metabolism, KLHL14 and KCNE3 as novel regulators of Epithelial-Mesenchymal Transition.

Scientific Reports
V Di LolloB Barboni

Abstract

Epithelial-mesenchymal transition (EMT) is a complex biological program between physiology and pathology. Here, amniotic epithelial cells (AEC) were used as in vitro model of transiently inducible EMT in order to evaluate the transcriptional insights underlying this process. Therefore, RNA-seq was used to identify the differentially expressed genes and enrichment analyses were carried out to assess the intracellular pathways involved. As a result, molecules exclusively expressed in AEC that experienced EMT (GSTA1-1 and GSTM3) or when this process is inhibited (KLHL14 and KCNE3) were identified. Lastly, the network theory was used to obtain a computational model able to recognize putative controller genes involved in the induction and in the prevention of EMT. The results suggested an opposite role of lysophosphatidic acid (LPA) synthesis and degradation enzymes in the regulation of EMT process. In conclusion, these molecules may represent novel EMT regulators and also targets for developing new therapeutic strategies.

References

Nov 1, 1992·American Journal of Medical Genetics·B BembiD Romeo
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·D R TaupinD K Podolsky
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Makoto KobayashiMasayuki Yamamoto
Oct 29, 2005·Journal of Cell Science·Réka Albert
Dec 13, 2006·The Journal of Biological Chemistry·Jimmy DonkorKaren Reue
Apr 3, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yuki MoritokiTooru Shimosegawa
May 6, 2008·Journal of Mammary Gland Biology and Neoplasia·David F Stern
Mar 3, 2009·Biochimica Et Biophysica Acta·David N BrindleyKaren Reue
Mar 18, 2009·Biochimica Et Biophysica Acta·Carl G GahmbergMikaela Grönholm
Mar 25, 2009·Bioinformatics·Jitao David Zhang, Stefan Wiemann
Apr 23, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Sarah J SantosSandra Z Haslam
Sep 3, 2009·The Journal of Physiology·Fiona O'MahonyBrian J Harvey
Feb 12, 2010·American Journal of Physiology. Cell Physiology·K A AromolaranE S Piedras-Rentería
Jul 22, 2010·Annual Review of Nutrition·Lauren S Csaki, Karen Reue
Feb 2, 2012·PloS One·Paul H van der HorstLeen J Blok
Mar 1, 2012·International Review of Cell and Molecular Biology·KangAe Lee, Celeste M Nelson
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Oct 4, 2012·Cell Transplantation·Antonia AlcarazFrancisco J Nicolás
Nov 24, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kristen J SkvorakStephen C Strom
Dec 29, 2012·Current Stem Cell Research & Therapy·Jing Yang TeeUrsula Manuelpillai
May 17, 2013·Human Genomics·Bajinder S DhanoaJames S Friedman
Dec 1, 2013·Stem Cells and Development·Stephen C StromToshio Miki
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Nov 7, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Xue-Diao PanLin-Quan Zang
Nov 16, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ganesh VenkatramanDavid N Brindley
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Apr 8, 2017·The Journal of Clinical Endocrinology and Metabolism·Carla JanzenGautam Chaudhuri

❮ Previous
Next ❯

Citations

Sep 18, 2020·International Journal of Molecular Sciences·Maria Rita CiteroniBarbara Barboni
Oct 30, 2020·Cancer Management and Research·Shunda WangYupei Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
nuclear translocation

Software Mentioned

edgeR
Cuffdiff
DESeq
topGO R - package
Bowtie2
Ensembl
Trimmomatic
Network Analyzer
mAEC
Cufflinks

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

JEMS : a Journal of Emergency Medical Services
Jon StudnekGregg Margolis
© 2021 Meta ULC. All rights reserved