Transcriptomic and epigenomics atlas of myotubes reveals insight into the circadian control of metabolism and development.

Epigenomics
Ali AltıntaşJuleen R Zierath

Abstract

Aim: Innate circadian rhythms are critical for optimal tissue-specific functions, including skeletal muscle, a major insulin-sensitive tissue responsible for glucose homeostasis. We determined whether transcriptional oscillations are associated with CpG methylation changes in skeletal muscle. Materials & methods: We performed rhythmicity analysis on the transcriptome and CpG methylome of circadian synchronized myotubes. Results: We identified several transcripts and CpG-sites displaying oscillatory behavior, which were enriched with Gene Ontology terms related to metabolism and development. Oscillating CpG methylation was associated with rhythmic expression of 31 transcripts. Conclusion: Although circadian oscillations may be regulated by rhythmic DNA methylation, strong rhythmic associations between transcriptome and CpG methylation were not identified. This resource constitutes a transcriptomic/epigenomic atlas of skeletal muscle and regulation of circadian rhythms.

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Citations

Apr 14, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Ferdinand Von WaldenKevin A Murach
Sep 18, 2020·The Journal of Physiology·Lewin SmallRomain Barrès
Dec 17, 2020·Epigenomics·Celeste Brady
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Apr 28, 2021·Nature Reviews. Genetics·Edward Saehong Oh, Art Petronis
May 29, 2021·Journal of Circadian Rhythms·Eric BarbatoThomas J Kelley

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Methods Mentioned

BETA
RNAseq
RNA-seq
PCR
RRBS

Software Mentioned

Trim Galore
Rsubread
featureCounts
Bismark
RAIN
GOrilla
Cutadapt

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