Transcriptomic and genomic structural variation analyses on grape cultivars reveal new insights into the genotype-dependent responses to water stress

Scientific Reports
C R CatacchioM F Cardone

Abstract

Grapevine (Vitis vinifera L.) is importantly cultivated worldwide for table grape and wine production. Its cultivation requires irrigation supply, especially in arid and semiarid areas. Water deficiency can affect berry and wine quality mostly depending on the extent of plant perceived stress, which is a cultivar-specific trait. We tested the physiological and molecular responses to water deficiency of two table grape cultivars, Italia and Autumn royal, and we highlighted their different adaptation. Microarray analyses revealed that Autumn royal reacts involving only 29 genes, related to plant stress response and ABA/hormone signal transduction, to modulate the response to water deficit. Instead, cultivar Italia orchestrates a very broad response (we found 1037 differentially expressed genes) that modifies the cell wall organization, carbohydrate metabolism, response to reactive oxygen species, hormones and osmotic stress. For the first time, we integrated transcriptomic data with cultivar-specific genomics and found that ABA-perception and -signalling are key factors mediating the varietal-specific behaviour of the early response to drought. We were thus able to isolate candidate genes for the genotype-dependent response to dr...Continue Reading

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Citations

Feb 2, 2021·Frontiers in Plant Science·Thanvi Srikant, Hajk-Georg Drost
Mar 23, 2021·Frontiers in Plant Science·Crista Ann BurbidgeCrystal Sweetman
Apr 4, 2021·International Journal of Molecular Sciences·Giorgio GambinoIvana Gribaudo

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Methods Mentioned

BETA
electrophoresis
Feature Extraction

Software Mentioned

MapMan
Cytoscape
STATISTICA
ClueGO App
Agilent Feature Extraction
SnpEff
Ensembl
limma
mrFAST
KbUS

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