Transcriptomic and metabonomic analyses reveal roles of VPS 29 in carotenoid accumulation in adductor muscles of QN Orange scallops.

Genomics
Junlin Song, Chunde Wang

Abstract

In our previous studies, we demonstrated that the accumulation of carotenoids in QN Orange scallops might be regulated by the vacuolar protein sorting 29 (VPS29) gene. VPS genes are involved in pigments accumulation (including carotenoids) in some species and VPS29 is known as the core component of the membrane transport complex Retromer. However, the possible mechanism of carotenoids accumulation underlying the VPS29 remains unexplored. This study aimed to further elucidate the roles of VPS29 in the carotenoid deposition. Transcriptomic analyses revealed four differentially expressed genes related to carotenoid accumulation, including three down-regulated genes, low-density lipoprotein receptor domain class, scavenger receptor, Niemann Pick C1-like 1, and one up-regulated gene, ATP binding cassette transporter in RNAi group. Results from metabonomic analyses indicated increased profiles of retinol and decreased fatty acids between the RNAi and the control group. It thus speculated that VPS may be related to the accumulation of carotenoids as RNAi of VPS 29 seemed to result in a reduction in pectenolone through the blockage in the absorption of carotenoids and an accelerated cleavage of carotenoids into retinol.

References

Jun 13, 2002·Journal of Agricultural and Food Chemistry·Mohd Nizar SallehMahinda Y Abeywardena
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cornelia KieferJohannes Von Lintig
Jul 24, 2007·Journal of Lipid Research·Alexandrine During, Earl H Harrison
Jan 12, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Glenn P LoboJohannes von Lintig
Sep 30, 2010·Traffic·Suzanne J NorwoodBrett M Collins
Feb 8, 2013·Traffic·Michael SchwakePaul Saftig
Mar 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Antonio Di MatteoAmalia Barone
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Mar 24, 2016·Biological Reviews of the Cambridge Philosophical Society·Suzanne T Williams
Feb 9, 2017·Trends in Genetics : TIG·David P L ToewsScott A Taylor
Feb 13, 2019·The Proceedings of the Nutrition Society·Torsten BohnPatrick Borel
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Leonie VenterJeremie Zander Lindeque

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