Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type.

Nature Neuroscience
Eszter BoldogGábor Tamás

Abstract

We describe convergent evidence from transcriptomics, morphology, and physiology for a specialized GABAergic neuron subtype in human cortex. Using unbiased single-nucleus RNA sequencing, we identify ten GABAergic interneuron subtypes with combinatorial gene signatures in human cortical layer 1 and characterize a group of human interneurons with anatomical features never described in rodents, having large 'rosehip'-like axonal boutons and compact arborization. These rosehip cells show an immunohistochemical profile (GAD1+CCK+, CNR1-SST-CALB2-PVALB-) matching a single transcriptomically defined cell type whose specific molecular marker signature is not seen in mouse cortex. Rosehip cells in layer 1 make homotypic gap junctions, predominantly target apical dendritic shafts of layer 3 pyramidal neurons, and inhibit backpropagating pyramidal action potentials in microdomains of the dendritic tuft. These cells are therefore positioned for potent local control of distal dendritic computation in cortical pyramidal neurons.

References

Jun 1, 1990·Brain : a Journal of Neurology·Z F KisvárdayP Somogyi
Apr 24, 1995·The Journal of Comparative Neurology·E A NimchinskyP R Hof
Jan 1, 1996·Hippocampus·T F Freund, G Buzsáki
Sep 1, 1997·Cerebral Cortex·Y Kawaguchi, Y Kubota
Mar 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qing XuStewart A Anderson
May 5, 2005·European Journal of Human Genetics : EJHG·Isabella BorgVera M Kalscheuer
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
Jun 24, 2008·Nature Reviews. Neuroscience·UNKNOWN Petilla Interneuron Nomenclature GroupRafael Yuste
Sep 5, 2008·PLoS Biology·Gábor MolnárGábor Tamás
Oct 24, 2008·Frontiers in Neural Circuits·Szabolcs OláhGábor Tamás
Mar 1, 2012·Science·Lucy M PalmerMatthew E Larkum
Apr 25, 2012·Annual Review of Neuroscience·István Katona, Tamás F Freund
Apr 28, 2012·Bioinformatics·David S DeLucaGad Getz
Jun 30, 2012·Bioinformatics·Liguo WangWei Li
Sep 22, 2012·Nature·Michael J HawrylyczAllan R Jones
Aug 21, 2013·PLoS Computational Biology·Michael LawrenceVincent J Carey
Oct 25, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthijs B VerhoogHuibert D Mansvelder
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rashel V GrindbergRoger S Lasken
Jan 5, 2014·Nature Protocols·Simone PicelliRickard Sandberg
Apr 4, 2014·Nature·Jeremy A MillerEd S Lein
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 6, 2014·ALTEX·Sarah E CavanaughNeal D Barnard
Jul 25, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juan MoralesRafael Yuste
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Oct 10, 2015·Cell·Henry MarkramFelix Schürmann
Jan 5, 2016·Nature Neuroscience·Bosiljka TasicHongkui Zeng

❮ Previous
Next ❯

Citations

Dec 14, 2018·BJPsych International
May 17, 2019·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Taiki Hara, Fumitaka Osakada
Jan 30, 2020·Journal of Neurophysiology·Kevin LeeJohanna M Montgomery
Mar 7, 2020·Human Molecular Genetics·Eric BrennerR Dayne Mayfield
Jul 11, 2019·Genome Biology·Isabel MendizabalSoojin V Yi
Jul 13, 2019·Annual Review of Neuroscience·Gord Fishell, Adam Kepecs
Jul 15, 2020·The European Journal of Neuroscience·Marco CapognaArianna Maffei
May 24, 2019·Science Translational Medicine·Elizabeth M C Fisher, David M Bannerman
May 28, 2019·Frontiers in Molecular Neuroscience·Lin QueCsaba Földy
May 13, 2020·Nature Reviews. Neuroscience·Panayiota Poirazi, Athanasia Papoutsi
Apr 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chia-Yu ChangTsung-Jung Ho
Sep 13, 2018·Nature Reviews. Neuroscience·Sian Lewis
Dec 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Adam J NorthcuttDavid J Schulz
Jun 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·August Yue HuangChristopher A Walsh
Oct 6, 2020·Epilepsy Currents·Vanesa Nieto-Estévez, Jenny Hsieh
Jun 4, 2019·Frontiers in Synaptic Neuroscience·Jean-Sébastien Jouhanneau, James F A Poulet
Jan 11, 2020·Frontiers in Cellular Neuroscience·Kanella Prodromidou, Rebecca Matsas
Feb 8, 2020·Nature Reviews. Neuroscience·Xiao-Jing Wang
Mar 15, 2019·Acta Neuropathologica Communications·Iris Margalit TrutzerBasilis Zikopoulos
Jan 4, 2020·Science·Albert GidonMatthew Evan Larkum
Mar 3, 2020·Frontiers in Synaptic Neuroscience·Guanxiao QiDirk Feldmeyer
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Guoping FengJohn H Morrison
Aug 19, 2020·Nature Neuroscience·Douglas Vormstein-SchneiderJordane Dimidschstein
Nov 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Stefano BertoGenevieve Konopka
May 20, 2020·Genome Research·Ekaterina KhrameevaPhilipp Khaitovich
Jul 19, 2020·Integrative and Comparative Biology·Yuxiang Liu, Genevieve Konopka
Dec 3, 2020·Cellular and Molecular Life Sciences : CMLS·Britt MossinkNael Nadif Kasri
Dec 30, 2020·ELife·Jeremy A MillerAmy Bernard
Jan 24, 2019·Cell Reports·William D ChronisterMichael J McConnell
Aug 2, 2019·Current Opinion in Cell Biology·Polina Kameneva, Igor Adameyko
Sep 27, 2019·Neuron·Cathryn R CadwellTomasz J Nowakowski
Nov 11, 2020·Neuroscience and Biobehavioral Reviews·Armin Raznahan, Christine M Disteche
Dec 17, 2020·Frontiers in Neurology·Quirijn P VerhoogErwin A van Vliet

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell-sorting
dissection
electron microscopy
PCR
RNA-seq
surgical resections

Software Mentioned

TOPHAT
Trimmomatic
RSEM
mathop
GenomicAlignments
limma 67 R package
R
R package
GenomicRanges
Reconstruct

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Allen Institute for Brain Science Network

The Allen Institute for Brain Science Network is a not-for-profit biomedical research organization that provides open access to multiple neuroscience tools and resources. Find the latest research from tjr Allen Institute for Brain Science Network here.