Transcriptomic and proteomic analyses on the supercooling ability and mining of antifreeze proteins of the Chinese white wax scale insect

Insect Science
Shu-Hui YuBo-Wen Liu

Abstract

The Chinese white wax scale insect, Ericerus pela, can survive at extremely low temperatures, and some overwintering individuals exhibit supercooling at temperatures below -30°C. To investigate the deep supercooling ability of E. pela, transcriptomic and proteomic analyses were performed to delineate the major gene and protein families responsible for the deep supercooling ability of overwintering females. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that genes involved in the mitogen-activated protein kinase, calcium, and PI3K-Akt signaling pathways and pathways associated with the biosynthesis of soluble sugars, sugar alcohols and free amino acids were dominant. Proteins responsible for low-temperature stress, such as cold acclimation proteins, glycerol biosynthesis-related enzymes and heat shock proteins (HSPs) were identified. However, no antifreeze proteins (AFPs) were identified through sequence similarity search methods. A random forest approach identified 388 putative AFPs in the proteome. The AFP gene ep-afp was expressed in Escherichia coli, and the expressed protein exhibited a thermal hysteresis activity of 0.97°C, suggesting its potential role in the deep s...Continue Reading

References

Mar 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·C KendziorskiM N Gould
Oct 22, 2005·Science·Laurie A Graham, Peter L Davies
Dec 1, 2005·Gene·Wensheng Qin, Virginia K Walker
Dec 22, 2006·Bioinformatics·Isabelle RivalsMarie-Claude Potier
Jul 27, 2007·Insect Molecular Biology·W QinV K Walker
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Jun 28, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Melody S Clark, M Roger Worland
Aug 20, 2008·Annual Review of Entomology·Le KangTong-Xian Liu
Dec 29, 2009·Trends in Plant Science·László Szabados, Arnould Savouré
Nov 9, 2010·Journal of Theoretical Biology·Krishna Kumar KandaswamyGanesan Pugalenthi
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas M TeetsDavid L Denlinger
Jun 27, 2013·BMC Genomics·Xin-Chao WangYa-Jun Yang
Apr 16, 2014·Journal of Theoretical Biology·Sukanta Mondal, Priyadarshini P Pai
Sep 5, 2014·Archives of Insect Biochemistry and Physiology·Pu Yang, Xiao-Ming Chen
Oct 24, 2014·Annual Review of Entomology·Allison M King, Thomas H MacRae

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.