Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage

PloS One
Alisha GuptaMuhammad Anzar

Abstract

Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the expanded blastocyst stage. Blastocysts developed from vitrified and slow-frozen morulae were subjected to microarray analysis and compared with blastocysts developed from unfrozen control morulae for differential gene expression. Morula to blastocyst conversion rate was higher (P < 0.05) in control (72%) and vitrified (77%) than in slow-frozen (34%) morulae. Total 20 genes were upregulated and 44 genes were downregulated in blastocysts developed from vitrified morulae (fold change ≥ ± 2, P < 0.05) in comparison with blastocysts developed from control morulae. In blastocysts developed from slow-frozen morulae, 102 genes were upregulated and 63 genes were downregulated (fold change ≥ ± 1.5, P < 0.05). Blastocysts developed from vitrified morulae exhibited significant changes in gene expression mainly involving embryo implantation (PTGS2, CALB1), lipid peroxidation and reactive oxygen species generation (HSD3B1, AKR1B1, APOA1) and cell differentiation (KRT19, CLDN23). However, blastocysts developed from slow-frozen morulae showed changes in the expression...Continue Reading

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Citations

Oct 1, 2020·International Journal of Molecular Sciences·Ximo Garcia-DominguezFrancisco Marco-Jiménez
Nov 7, 2020·Molecular Reproduction and Development·Thamiris V MarsicoMateus J Sudano
Jan 31, 2021·International Journal of Molecular Sciences·Cristina CuelloEmilio A Martinez
Dec 7, 2021·Frontiers in Veterinary Science·Cristina CuelloEmilio A Martinez

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Datasets Mentioned

BETA
GSE45381
GSE95382

Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

Ingenuity Pathway Analysis ( IPA )
Gydle [UNK]
MIAME
SAS Enterprise Guide
Pro [UNK]
Ingenuity
Excel
SAS
IPA
Array

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