Transcriptomic Features of Bovine Blastocysts Derived by Somatic Cell Nuclear Transfer

G3 : Genes - Genomes - Genetics
Byungkuk MinYong-Kook Kang

Abstract

Reprogramming incompletely occurs in most somatic cell nuclear transfer (SCNT) embryos, which results in misregulation of developmentally important genes and subsequent embryonic malfunction and lethality. Here we examined transcriptome profiles in single bovine blastocysts derived by in vitro fertilization (IVF) and SCNT. Different types of donor cells, cumulus cell and ear-skin fibroblast, were used to derive cSCNT and fSCNT blastocysts, respectively. SCNT blastocysts expressed 13,606 genes on average, similar to IVF (13,542). Correlation analysis found that both cSCNT and fSCNT blastocyst groups had transcriptomic features distinctive from the IVF group, with the cSCNT transcriptomes closer to the IVF ones than the fSCNT. Gene expression analysis identified 56 underrepresented and 78 overrepresented differentially expressed genes in both SCNT groups. A 400-kb locus harboring zinc-finger protein family genes in chromosome 18 were found coordinately down-regulated in fSCNT blastocysts, showing a feature of reprogramming-resistant regions. Probing into different categories of genes important for blastocyst development revealed that genes involved in trophectoderm development frequently were underrepresented, and those encoding ...Continue Reading

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Citations

Apr 7, 2019·Biology Open·Viju Vijayan PillaiVimal Selvaraj
Jul 16, 2020·Nucleic Acids Research·Byungkuk MinYong-Kook Kang
Mar 16, 2016·G3 : Genes - Genomes - Genetics·Byungkuk MinYong-Kook Kang
Sep 14, 2021·Biology of Reproduction·Jocelyn M CuthbertAbby D Benninghoff

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Methods Mentioned

BETA
RNA-seq
PCR

Software Mentioned

Integrated Genome Viewer ( IGV
HTSeq
DESeq
edgeR
TopHat
ENSEMBL
Circos

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